ArticlePloS one2013
Functional cooperation between vitamin D receptor and Runx2 in vitamin D-induced vascular calcification.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Effect of high-dose vitamin D supplementation on peripheral arterial calcification: secondary analysis of a randomized controlled trial.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2020Trial
- Apolipoprotein C3 exacerbates vascular calcification by promoting ferroptosis via the TLR2/AMPK pathway in vascular smooth muscle cells.Redox biology · 2026Article
- Synergistic effects of dexamethasone and vitamins D and K on mesenchymal stem cell differentiation.Biomedical engineering online · 2026Article
- Considerations in the Use of Mouse Models of Vascular and Valvular Calcification.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- Review
- In Vitro Models of Cardiovascular Calcification.Biomedicines · 2024Review
- Multiomics profiling reveals VDR as a central regulator of mesenchymal stem cell senescence with a known association with osteoporosis after high-fat diet exposure.International journal of oral science · 2024Article
- Vitamin D3 Release from MgO Doped 3D Printed TCP Scaffolds for Bone Regeneration.ACS biomaterials science & engineering · 2024Article
- Flavocoxid Ameliorates Aortic Calcification Induced by Hypervitaminosis DCardiovascular drugs and therapy · 2022Article
- Association of vitamin D status with COVID-19 and its severity : Vitamin D and COVID-19: a narrative review.Reviews in endocrine & metabolic disorders · 2022Review
- DNA Polymerase Gamma Recovers Mitochondrial Function and Inhibits Vascular Calcification by Interacted with p53.International journal of biological sciences · 2022Article
- Klotho deficiency-induced arterial calcification involves osteoblastic transition of VSMCs and activation of BMP signaling.Journal of cellular physiology · 2022Article
- Potential impact of the steroid hormone, vitamin D, on the vasculature.American heart journal · 2021Review
- Roles of Nuclear Receptors in Vascular Calcification.International journal of molecular sciences · 2021Review
- Do Interactions of Vitamin DCurrent osteoporosis reports · 2021Review
- Calciprotein Particles: Balancing Mineral Homeostasis and Vascular Pathology.Arteriosclerosis, thrombosis, and vascular biology · 2021Review
- Screening of an Epigenetic Drug Library Identifies 4-((hydroxyamino)carbonyl)-International journal of molecular sciences · 2020Article
- Vitamin D and cardiovascular disorders.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2019Review
- Regulation of calcific vascular and valvular disease by nuclear receptors.Current opinion in lipidology · 2019Review
- Skeletal and Extraskeletal Actions of Vitamin D: Current Evidence and Outstanding Questions.Endocrine reviews · 2019Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transdifferentiation of vascular smooth muscle cells (VSMCs) into osteoblast-like cells has been implicated in the context of vascular calcification. We investigated the roles of vitamin D receptor (Vdr) and runt-related transcription factor 2 (Runx2) in the osteoblastic differentiation of VSMCs in response to vitamin D3 using in vitro VSMCs cultures and in vivo in Vdr knockout (Vdr(-/-)) and Runx2 carboxy-terminus truncated heterozygous (Runx2(+/ΔC)) mice. Treatment of VSMCs with active vitamin D3 promoted matrix mineral deposition, and increased the expressions of Vdr, Runx2, and of osteoblastic genes but decreased the expression of smooth muscle myosin heavy chain in primary VSMCs cultures. Immunoprecipitation experiments suggested an interaction between Vdr and Runx2. Furthermore, silencing Vdr or Runx2 attenuated the procalcific effects of vitamin D3. Functional cooperation between Vdr and Runx2 in vascular calcification was also confirmed in in vivo mouse models. Vascular calcification induced by high-dose vitamin D3 was completely inhibited in Vdr(-/-) or Runx2(+/ΔC) mice, despite elevated levels of serum calcium or alkaline phosphatase. Collectively, these findings suggest that functional cooperation between Vdr and Runx2 is necessary for vascular calcification in response to vitamin D3.
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