ArticlePloS one2013
Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- ABC-type salt tolerance transporter genes are abundant and mutually shared among the microorganisms of the hypersaline Sambhar Lake.Extremophiles : life under extreme conditions · 2025Article
- An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food-Microbiota-Host Interactions in the Human Gut.International journal of molecular sciences · 2023Review
- New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.Communications biology · 2023Article
- Isolation of novel cold-tolerance genes from rhizosphere microorganisms of Antarctic plants by functional metagenomics.Frontiers in microbiology · 2022Article
- Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile.Frontiers in microbiology · 2021Article
- Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments.Frontiers in microbiology · 2020Article
- Molecular and Biochemical Characterization of Salt-Tolerant Trehalose-6-Phosphate Hydrolases Identified by Screening and Sequencing Salt-Tolerant Clones From the Metagenomic Library of the Gastrointestinal Tract.Frontiers in microbiology · 2020Article
- Prospecting for microbial α-The Journal of biological chemistry · 2019Article
- Molecular Characterization of Circulating Microbiome Signatures in Rheumatoid Arthritis.Frontiers in cellular and infection microbiology · 2019Article
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
- Metagenomic Profiling of Soil Microbes to Mine Salt Stress Tolerance Genes.Frontiers in microbiology · 2018Article
- Gut microbiota and the development of pediatric diseases.Journal of gastroenterology · 2015Review
- Under the microscope: From pathogens to probiotics and back.Bioengineered · 2015Review
- Current functional metagenomic approaches only expand the existing protease sequence space, but does not presently add any novelty to it.Current microbiology · 2015Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
- Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment.Frontiers in microbiology · 2015Article
- Article
- Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome.Frontiers in microbiology · 2014Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment.
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