ArticlePloS one2013
Protein transfection study using multicellular tumor spheroids of human hepatoma Huh-7 cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Development ofMolecules (Basel, Switzerland) · 2024Article
- Utilization of HEPES for Enhancing Protein Transfection into Mammalian Cells.Molecular therapy. Methods & clinical development · 2019Article
- Visualizing the cellular route of entry of a cystine-knot peptide with Xfect transfection reagent by electron microscopy.Scientific reports · 2019Article
- Cellular uptake of a cystine-knot peptide and modulation of its intracellular trafficking.Scientific reports · 2016Article
- A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery.Scientific reports · 2016Article
- Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells.Molecular and cellular biochemistry · 2015Article
- Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function.AMB Express · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several protein transfection reagents are commercially available and are powerful tools for elucidating function of a protein in a cell. Here we described protein transfection studies of the commercially available reagents, Pro-DeliverIN, Xfect, and TuboFect, using Huh-7 multicellular tumor spheroid (MCTS) as a three-dimensional in vitro tumor model. A cellular uptake study using specific endocytosis inhibitors revealed that each reagent was internalized into Huh-7 MCTS by different mechanisms, which were the same as monolayer cultured Huh-7 cells. A certain amount of Pro-DeliverIN and Xfect was uptaken by Huh-7 cells through caveolae-mediated endocytosis, which may lead to transcytosis through the surface-first layered cells of MCTS. The results presented here will help in the choice and use of protein transfection reagents for evaluating anti-tumor therapeutic proteins against MCTS models.
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Registered trials
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