ArticlePloS one2013
Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- SMCHD1 is a target for gene activation therapy to treat Prader-Willi syndrome.Science advances · 2026Article
- High frequency of germline recombination in Nestin-Cre transgenic mice crossed with Glucagon-like peptide 1 receptor floxed mice.PloS one · 2023Article
- Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in theCells · 2022Article
- Article
- Brain-specific PAPP-A knock-out mice?Experimental gerontology · 2021Article
- Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background.Scientific reports · 2021Article
- Deviant reporter expression and P2X4 passenger gene overexpression in the soluble EGFP BAC transgenic P2X7 reporter mouse model.Scientific reports · 2020Article
- Article
- Ectopic germline recombination activity of the widely used Foxp3-YFP-Cre mouse: a case report.Immunology · 2020Article
- Off-Target Deletion of ConditionalCells · 2019Article
- Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.eLife · 2018Article
- Comparison of folate-conjugated rapamycin versus unconjugated rapamycin in an orthologous mouse model of polycystic kidney disease.American journal of physiology. Renal physiology · 2018Article
- Ste20-like kinase, SLK, a novel mediator of podocyte integrity.American journal of physiology. Renal physiology · 2018Article
- Connexin 43 Controls the Astrocyte Immunoregulatory Phenotype.Brain sciences · 2018Article
- Connexin-43 Gap Junctions Are Responsible for the Hypothalamic Tanycyte-Coupled Network.Frontiers in cellular neuroscience · 2018Article
- Cre-Recombinase Dependent Germline Deletion of a Conditional Allele in the Rgs9cre Mouse Line.Frontiers in neural circuits · 2018Article
- A Role for Sox2 in the Adult Cerebellum.Journal of stem cell research & therapy · 2018Article
- Male germline recombination of a conditional allele by the widely used Dermo1-cre (Twist2-cre) transgene.Genesis (New York, N.Y. : 2000) · 2017Article
- Genetic modifiers as relevant biological variables of eye disorders.Human molecular genetics · 2017Review
- Pejvakin, a Candidate Stereociliary Rootlet Protein, Regulates Hair Cell Function in a Cell-Autonomous Manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ-line recombination mediated by the hGFAP-Cre and nestin-Cre transgenes. Such control is essential to avoid pleiotropic effects due to germ-line deletion of loxP-flanked target genes and to maintain the CNS-restricted deletion status in transgenic mouse colonies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.