ArticlePloS one2013
Skeletal cell differentiation is enhanced by atmospheric dielectric barrier discharge plasma treatment.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Enhancing Tissue Integration and Reducing Inflammation in Silicone and Human Acellular Dermal Matrix Implants via Vacuum Plasma Treatment.International journal of molecular sciences · 2025Article
- Evaluating the Healing Potential of J-Plasma Scalpel-Created Surgical Incisions in Porcine and Rat Models.Biomedicines · 2024Article
- Effect of Cold Atmospheric Plasma (CAP) on Osteogenic Differentiation Potential of Human Osteoblasts.International journal of molecular sciences · 2022Article
- A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells.Pharmaceutics · 2022Article
- Biocompatible Gas Plasma Treatment Affects Secretion Profiles but Not Osteogenic Differentiation in Patient-Derived Mesenchymal Stromal Cells.International journal of molecular sciences · 2022Article
- Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells.Biomedicines · 2021Article
- Non-thermal atmospheric pressure plasma activates Wnt/β-catenin signaling in dermal papilla cells.Scientific reports · 2021Article
- Cold Atmospheric Plasma: A New Strategy Based Primarily on Oxidative Stress for Osteosarcoma Therapy.Journal of clinical medicine · 2021Review
- Non-Thermal Plasma-Induced Immunogenic Cell Death in Cancer: A Topical Review.Journal of physics D: Applied physics · 2019Article
- Non-Thermal Plasma Accelerates Astrocyte Regrowth and Neurite Regeneration Following Physical Trauma In Vitro.Applied sciences (Basel, Switzerland) · 2019Article
- Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality.Scientific reports · 2019Article
- Promotion of osteogenic differentiation by non-thermal biocompatible plasma treated chitosan scaffold.Scientific reports · 2019Article
- Regulation of Redox Homeostasis by Nonthermal Biocompatible Plasma Discharge in Stem Cell Differentiation.Oxidative medicine and cellular longevity · 2019Review
- Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens.International journal of molecular sciences · 2018Article
- Non-thermal plasma treatment improves chicken sperm motility via the regulation of demethylation levels.Scientific reports · 2018Article
- Cold atmospheric helium plasma causes synergistic enhancement in cell death with hyperthermia and an additive enhancement with radiation.Scientific reports · 2017Article
- Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress.International journal of molecular sciences · 2017Article
- Time-dependent effects of ultraviolet and nonthermal atmospheric pressure plasma on the biological activity of titanium.Scientific reports · 2016Article
- Chemical modification of extracellular matrix by cold atmospheric plasma-generated reactive species affects chondrogenesis and bone formation.Journal of tissue engineering and regenerative medicine · 2016Article
- Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation.Cytotechnology · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Enhancing chondrogenic and osteogenic differentiation is of paramount importance in providing effective regenerative therapies and improving the rate of fracture healing. This study investigated the potential of non-thermal atmospheric dielectric barrier discharge plasma (NT-plasma) to enhance chondrocyte and osteoblast proliferation and differentiation. Although the exact mechanism by which NT-plasma interacts with cells is undefined, it is known that during treatment the atmosphere is ionized generating extracellular reactive oxygen and nitrogen species (ROS and RNS) and an electric field. Appropriate NT-plasma conditions were determined using lactate-dehydrogenase release, flow cytometric live/dead assay, flow cytometric cell cycle analysis, and Western blots to evaluate DNA damage and mitochondrial integrity. We observed that specific NT-plasma conditions were required to prevent cell death, and that loss of pre-osteoblastic cell viability was dependent on intracellular ROS and RNS production. To further investigate the involvement of intracellular ROS, fluorescent intracellular dyes Mitosox (superoxide) and dihydrorhodamine (peroxide) were used to assess onset and duration after NT-plasma treatment. Both intracellular superoxide and peroxide were found to increase immediately post NT-plasma treatment. These increases were sustained for one hour but returned to control levels by 24 hr. Using the same treatment conditions, osteogenic differentiation by NT-plasma was assessed and compared to peroxide or osteogenic media containing β-glycerolphosphate. Although both NT-plasma and peroxide induced differentiation-specific gene expression, neither was as effective as the osteogenic media. However, treatment of cells with NT-plasma after 24 hr in osteogenic or chondrogenic media significantly enhanced differentiation as compared to differentiation media alone. The results of this study show that NT-plasma can selectively initiate and amplify ROS signaling to enhance differentiation, and suggest this technology could be used to enhance bone fusion and improve healing after skeletal injury.
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