ArticlePloS one2013
Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
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- Temporal dynamics of stress response in Halomonas elongata to NaCl shock: physiological, metabolomic, and transcriptomic insights.Microbial cell factories · 2024Article
- Mechanism of Salt Tolerance and Plant Growth Promotion inInternational journal of molecular sciences · 2023Article
- Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates.BMC biotechnology · 2023Article
- Stress-induced activation of the proline biosynthetic pathway in Bacillus subtilis: a population-wide and single-cell study of the osmotically controlled proHJ promoter.Microbial biotechnology · 2022Article
- Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation inSynthetic and systems biotechnology · 2022Article
- L-Proline Synthesis Mutants ofFrontiers in microbiology · 2022Article
- Antibiotic tolerance in environmentally stressedmicroLife · 2022Article
- Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea.BMC microbiology · 2021Article
- Exploitation of ammonia-inducible promoters for enzyme overexpression in Bacillus licheniformis.Journal of industrial microbiology & biotechnology · 2021Article
- The organosulfur compound dimethylsulfoniopropionate (DMSP) is utilized as an osmoprotectant byApplied and environmental microbiology · 2021Article
- Enhanced Glutamate Synthesis and Export by the Thermotolerant Emerging Industrial WorkhorseFrontiers in microbiology · 2021Article
- Degeneration of industrial bacteria caused by genetic instability.World journal of microbiology & biotechnology · 2020Review
- Peeling the Layers Away: The Genomic Characterization ofFrontiers in microbiology · 2020Article
- Metabolic Rearrangements Causing Elevated Proline and Polyhydroxybutyrate Accumulation During the Osmotic Adaptation Response ofFrontiers in bioengineering and biotechnology · 2020Article
- Transcriptional Changes in the Xylose Operon inInternational journal of molecular sciences · 2019Article
- In silico exploration of Red Sea Bacillus genomes for natural product biosynthetic gene clusters.BMC genomics · 2018Article
- Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum.Journal of microbiology (Seoul, Korea) · 2017Article
- Cell-Biological Studies of Osmotic Shock Response in Streptomyces spp.Journal of bacteriology · 2017Article
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Authors and funding
17 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.
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