ArticleStem cells international2013
Differentiation of dental pulp stem cells into neuron-like cells in serum-free medium.
Article in Stem cells international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Facial Nerve Regeneration in Immunodeficient Rats Using a Bio 3D Conduit Fabricated From Human Dental Pulp Stem Cells.Stem cells international · 2025Article
- Photosensitive Hydrogels Encapsulating DPSCs and AgNPs for Dental Pulp Regeneration.International dental journal · 2024Article
- The temporospatial relationship between mouse dental pulp stem cells and tooth innervation.Journal of dental sciences · 2024Article
- Potential of dental pulp stem cells and their products in promoting peripheral nerve regeneration and their future applications.World journal of stem cells · 2023Review
- Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS.Frontiers in pediatrics · 2023Article
- Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?International journal of molecular sciences · 2022Review
- Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism.Biomedicines · 2022Article
- Potential role of dental pulp stem cells conditioned medium for odontoblastic differentiation.Biological research · 2022Article
- Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins.Frontiers in cell and developmental biology · 2022Article
- Article
- The potential roles of dental pulp stem cells in peripheral nerve regeneration.Frontiers in neurology · 2022Review
- Rapid differentiation of human dental pulp stem cells to neuron-like cells by high KBiophysics and physicobiology · 2020Article
- Autophagy in dental tissues: a double-edged sword.Cell death & disease · 2016Article
- Promoting effect of small molecules in cardiomyogenic and neurogenic differentiation of rat bone marrow-derived mesenchymal stem cells.Drug design, development and therapy · 2016Article
- Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells.Stem cells and cloning : advances and applications · 2016Article
- Immunophenotypic and Molecular Analysis of Human Dental Pulp Stem Cells Potential for Neurogenic Differentiation.Contemporary clinical dentistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics of Cd146(+) , Cd166(+) , and Cd31(-) in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation of Nestin was observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereas Tub3 was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.