ArticlePLoS pathogens2013
The malarial serine protease SUB1 plays an essential role in parasite liver stage development.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Peptidic Boronic AcidJournal of medicinal chemistry · 2024Article
- Prodomain-driven enzyme dimerization: a pH-dependent autoinhibition mechanism that controlsmBio · 2024Article
- Peptidic boronic acids are potent cell-permeable inhibitors of the malaria parasite egress serine protease SUB1.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Malaria parasite egress at a glance.Journal of cell science · 2021Review
- A Plasmodium cysteine protease required for efficient transition from the liver infection stage.PLoS pathogens · 2020Article
- Overlapping and distinct roles of CDPK family members in the pre-erythrocytic stages of the rodent malaria parasite, Plasmodium berghei.PLoS pathogens · 2020Article
- A malaria parasite subtilisin propeptide-like protein is a potent inhibitor of the egress protease SUB1.The Biochemical journal · 2020Article
- Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development.Malaria journal · 2019Article
- The Plasmodium berghei serine protease PbSUB1 plays an important role in male gamete egress.Cellular microbiology · 2019Article
- Structural Insights Into KeyFrontiers in microbiology · 2019Review
- A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress.Science (New York, N.Y.) · 2017Article
- Proteases as antimalarial targets: strategies for genetic, chemical, and therapeutic validation.The FEBS journal · 2017Review
- Article
- The Activity of a Hexameric M17 Metallo-Aminopeptidase Is Associated With Survival ofFrontiers in microbiology · 2017Article
- In silico study of subtilisin-like protease 1 (SUB1) from different Plasmodium species in complex with peptidyl-difluorostatones and characterization of potent pan-SUB1 inhibitors.Journal of molecular graphics & modelling · 2016Article
- Advances in molecular genetic systems in malaria.Nature reviews. Microbiology · 2015Review
- A genome-scale vector resource enables high-throughput reverse genetic screening in a malaria parasite.Cell host & microbe · 2015Article
- A Plasmodium phospholipase is involved in disruption of the liver stage parasitophorous vacuole membrane.PLoS pathogens · 2015Article
- PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites.Nucleic acids research · 2015Article
- Conditional U1 Gene Silencing in Toxoplasma gondii.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Transmission of the malaria parasite to its vertebrate host involves an obligatory exoerythrocytic stage in which extensive asexual replication of the parasite takes place in infected hepatocytes. The resulting liver schizont undergoes segmentation to produce thousands of daughter merozoites. These are released to initiate the blood stage life cycle, which causes all the pathology associated with the disease. Whilst elements of liver stage merozoite biology are similar to those in the much better-studied blood stage merozoites, little is known of the molecular players involved in liver stage merozoite production. To facilitate the study of liver stage biology we developed a strategy for the rapid production of complex conditional alleles by recombinase mediated engineering in Escherichia coli, which we used in combination with existing Plasmodium berghei deleter lines expressing Flp recombinase to study subtilisin-like protease 1 (SUB1), a conserved Plasmodium serine protease previously implicated in blood stage merozoite maturation and egress. We demonstrate that SUB1 is not required for the early stages of intrahepatic growth, but is essential for complete development of the liver stage schizont and for production of hepatic merozoites. Our results indicate that inhibitors of SUB1 could be used in prophylactic approaches to control or block the clinically silent pre-erythrocytic stage of the malaria parasite life cycle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.