ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2014
In situ intracellular calcium oscillations in osteocytes in intact mouse long bones under dynamic mechanical loading.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 110 citations in OpenAlex.
- Intravital Depletion of Cholesterol Reduces Membrane Dynamics and Increases Mechanosensitivity in Osteocytes In Vivo.bioRxiv : the preprint server for biology · 2026Article
- Real-Time Axial Motion Compensation for Intravital Two-Photon Imaging of Mechanically Loaded Bone.bioRxiv : the preprint server for biology · 2026Article
- Fibrin, from blood to bone: a review.Bone reports · 2026Review
- Intravital Microscopy of Osteocytes: A New Era in Bone Cell Biology.Current osteoporosis reports · 2025Review
- Combined Effects of Mechanical Loading and Piezo1 Chemical Activation on 22-Months-Old Female Mouse Bone Adaptation.Aging cell · 2025Article
- Numerical study of interstitial fluid flow behavior in osteons under dynamic loading.BMC musculoskeletal disorders · 2025Article
- Culture system for longitudinal monitoring of bone dynamics ex vivo.Biotechnology and bioengineering · 2025Article
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- Computational modeling based on confocal imaging predicts changes in osteocyte and dendrite shear stress due to canalicular loss with aging.Biomechanics and modeling in mechanobiology · 2024Article
- Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics.Nature communications · 2024Article
- Oscillating Fluid Flow Activated Osteocyte Lysate-Based Hydrogel for Regulating Osteoblast/Osteoclast Homeostasis to Enhance Bone Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes.Journal of visualized experiments : JoVE · 2023Article
- Glucose- and glutamine-dependent bioenergetics sensitize bone mechanoresponse after unloading by modulating osteocyte calcium dynamics.The Journal of clinical investigation · 2023Article
- Mechanical properties of trabeculae and osteocyte morphology change significantly in different areas of the necrotic femoral head.Frontiers in cell and developmental biology · 2023Article
- Mechanosensitive piezo1 calcium channel activates connexin 43 hemichannels through PI3K signaling pathway in bone.Cell & bioscience · 2022Article
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- Nested information processing in the living world.Annals of the New York Academy of Sciences · 2021Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Osteocytes have been hypothesized to be the major mechanosensors in bone. How in situ osteocytes respond to mechanical stimuli is still unclear because of technical difficulties. In vitro studies have shown that osteocytes exhibited unique calcium (Ca(2+)) oscillations to fluid shear. However, whether this mechanotransduction phenomenon holds for in situ osteocytes embedded within a mineralized bone matrix under dynamic loading remains unknown. Using a novel synchronized loading/imaging technique, we successfully visualized in real time and quantified Ca(2+) responses in osteocytes and bone surface cells in situ under controlled dynamic loading on intact mouse tibia. The resultant fluid-induced shear stress on the osteocyte in the lacunocanalicular system (LCS) was also quantified. Osteocytes, but not surface cells, displayed repetitive Ca(2+) spikes in response to dynamic loading, with spike frequency and magnitude dependent on load magnitude, tissue strain, and shear stress in the LCS. The Ca(2+) oscillations were significantly reduced by endoplasmic reticulum (ER) depletion and P2 purinergic receptor (P2R)/phospholipase C (PLC) inhibition. This study provides direct evidence that osteocytes respond to in situ mechanical loading by Ca(2+) oscillations, which are dependent on the P2R/PLC/inositol trisphosphate/ER pathway. This study develops a novel approach in skeletal mechanobiology and also advances our fundamental knowledge of bone mechanotransduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.