ArticleeLife2013
A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution.
Article in eLife, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Tangled Tail of Mechanically Interlocked Peptides.Journal of the American Chemical Society · 2026Review
- Highly stable bacteriophages PIN1 and PIN2 have hallmarks of flagellotropic phages but infect immotile bacteria.Npj viruses · 2025Article
- Cryo-EM structure of cyanopodophage A4 reveals a pentameric pre-ejectosome in the double-stabilized capsid.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Stabilization mechanism accommodating genome length variation in evolutionarily related viral capsids.Nature communications · 2025Article
- Proteomic Analysis of Marine Bacteriophages: Structural Conservation, Post-Translational Modifications, and Phage-Host Interactions.Environmental microbiology · 2025Article
- Characterisation of NewViruses · 2024Article
- pyCapsid: identifying dominant dynamics and quasi-rigid mechanical units in protein shells.Bioinformatics (Oxford, England) · 2024Article
- Structure and proposed DNA delivery mechanism of a marine roseophage.Nature communications · 2023Article
- A structural dendrogram of the actinobacteriophage major capsid proteins provides important structural insights into the evolution of capsid stability.Structure (London, England : 1993) · 2023Article
- A Capsid Structure ofViruses · 2022Article
- Predicting the capsid architecture of phages from metagenomic data.Computational and structural biotechnology journal · 2022Article
- Capsid Structure ofJournal of virology · 2021Article
- Efficient Flexible Fitting Refinement with Automatic Error Fixing for De Novo Structure Modeling from Cryo-EM Density Maps.Journal of chemical information and modeling · 2021Article
- Cryo-EM structure ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Human Gut Phageome: Origins and Roles in the Human Gut Microbiome.Frontiers in cellular and infection microbiology · 2021Review
- Structures of capsid and capsid-associated tegument complex inside the Epstein-Barr virus.Nature microbiology · 2020Article
- Cryo-EM structure of the varicella-zoster virus A-capsid.Nature communications · 2020Article
- The architecture and stabilisation of flagellotropic tailed bacteriophages.Nature communications · 2020Article
- Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins.Viruses · 2020Article
- Evolving a Peptide: Library Platforms and Diversification Strategies.International journal of molecular sciences · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Bacteriophage BPP-1 infects and kills Bordetella species that cause whooping cough. Its diversity-generating retroelement (DGR) provides a naturally occurring phage-display system, but engineering efforts are hampered without atomic structures. Here, we report a cryo electron microscopy structure of the BPP-1 head at 3.5 Å resolution. Our atomic model shows two of the three protein folds representing major viral lineages: jellyroll for its cement protein (CP) and HK97-like ('Johnson') for its major capsid protein (MCP). Strikingly, the fold topology of MCP is permuted non-circularly from the Johnson fold topology previously seen in viral and cellular proteins. We illustrate that the new topology is likely the only feasible alternative of the old topology. β-sheet augmentation and electrostatic interactions contribute to the formation of non-covalent chainmail in BPP-1, unlike covalent inter-protein linkages of the HK97 chainmail. Despite these complex interactions, the termini of both CP and MCP are ideally positioned for DGR-based phage-display engineering. DOI: http://dx.doi.org/10.7554/eLife.01299.001.
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