ArticleTranslational stroke research2014
Characterization of a new model of thromboembolic stroke in C57 black/6J mice.
Article in Translational stroke research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Efficacy of Alteplase in a Mouse Model of Acute Ischemic Stroke: A Retrospective Pooled Analysis.Stroke · 2016Pooled it
- Nanomedicines and stroke: Advantages in chronic inflammation treatment and neural regeneration.Neural regeneration research · 2026Article
- Review
- MRI-based microthrombi detection in stroke with polydopamine iron oxide.Nature communications · 2024Article
- Preclinical models of middle cerebral artery occlusion: new imaging approaches to a classic technique.Frontiers in neurology · 2023Review
- Development of a carotid artery thrombolysis stroke model in mice.Blood advances · 2022Article
- Optimisation of a Mouse Model of Cerebral Ischemia-Reperfusion to Address Issues of Survival and Model Reproducibility and Consistency.Computational intelligence and neuroscience · 2022Article
- A Novel Thromboplastin-Based Rat Model of Ischemic Stroke.Brain sciences · 2021Article
- Development and Testing of Thrombolytics in Stroke.Journal of stroke · 2021Review
- Filling the gaps on stroke research: Focus on inflammation and immunity.Brain, behavior, and immunity · 2021Review
- A review of experimental models of focal cerebral ischemia focusing on the middle cerebral artery occlusion model.F1000Research · 2021Review
- Implication of MicroRNA503 in Brain Endothelial Cell Function and Ischemic Stroke.Translational stroke research · 2020Article
- Transient versus Permanent MCA Occlusion in Mice Genetically Modified to Have Good versus Poor Collaterals.Med one · 2019Article
- Focal Ischaemic Infarcts Expand Faster in Cerebellar Cortex than Cerebral Cortex in a Mouse Photothrombotic Stroke Model.Translational stroke research · 2018Article
- Partial MHC class II constructs as novel immunomodulatory therapy for stroke.Neurochemistry international · 2017Review
- A Post-stroke Therapeutic Regimen with Omega-3 Polyunsaturated Fatty Acids that Promotes White Matter Integrity and Beneficial Microglial Responses after Cerebral Ischemia.Translational stroke research · 2016Article
- A novel mouse model of thromboembolic stroke.Journal of neuroscience methods · 2015Review
- Animal models of ischemic stroke and their application in clinical research.Drug design, development and therapy · 2015Review
- Anti-inflammatory signaling: the point of convergence for medical gases in neuroprotection against ischemic stroke.Medical gas researchReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study characterizes a new model of thromboembolic stroke of the middle cerebral artery in C57 black/6J mice, thus offering an opportunity to use the model for studying ischemic stroke in transgenic mice. Thromboembolic stroke was induced by local injection of either 1.5 or 3.0 UI of thrombin directly into the right MCA of C57 black/6J mice. Cerebral blood flow (CBF) velocity was measured continuously by laser Doppler flowmetry, which allowed documentation of both MCA occlusion and of spontaneous recanalization. After 24 h, all animals were euthanized. Cryosections were cut at 400-μm intervals and silver stained with the high-contrast method for volumetric assessment of infarct size. Interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), caspase-3 and hsp 70 protein levels were investigated by immunofluorescence. Thrombin injection resulted in clot formation in all animals. Cortical infarction occurred in 63% of the mice while 37% had a spontaneous MCA recanalization during the first 20 min following thrombin injection. In cases of successful MCA occlusion with consequent infarction, the clot was stable up to 2 h after formation. Subsequently, 20% recanalized spontaneously. Infarctions were restricted to the cortex with a mean lesion volume of 36 ± 5 for 1.5 UI and 56 ± 8 for 3.0 UI thrombin. Protein levels of IL-6, TNF-α, caspase-3, and hsp 70 were significantly increased after MCAO. The results demonstrate that the mouse thromboembolic stroke model produces cortical infarctions of consistent size in C57 black/6J mice, which is dependent upon the amount of thrombin used for clot formation. Spontaneous MCA recanalization occurs after 2 h of ischemia in 20% of mice. Thus, the thromboembolic model is an applicable stroke model for C57 black/6J mice, which mimics many of the features of human stroke, including spontaneous recanalization. However, strain differences between Swiss and C57 black/6J mice must be taken into account when using the model.
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