ArticleThe Journal of biological chemistry2014
Ascorbate efflux as a new strategy for iron reduction and transport in plants.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 172 citations in OpenAlex.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Iron-ascorbate complex formation and redox behavior in Tris buffer under aerobic and anaerobic conditions: relevance to iron-deficiency anemia.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Beyond R-Genes: Dissecting Metabolic and Nutrient-Driven Wheat Rust Resistance Through Induced Mutagenesis.Plants (Basel, Switzerland) · 2026Review
- Phenotypic and molecular analysis of seabuckthorn accessions reveal promising genotypes and candidate genes associated with micronutrients.BMC plant biology · 2026Article
- Regulatory Effects of Different Doses of Penoxsulam on Endogenous Hormones and Antioxidant System in Foxtail Millet.Plants (Basel, Switzerland) · 2025Article
- Origin and evolution of signaling pathways responsible for ascorbic acid synthesis and catabolism during plant terrestrialization.Horticulture research · 2025Article
- Toward Nutrient-Rich Rice: Biofortification through Mineral Accumulation and Low Phytic Acid Content.Journal of agricultural and food chemistry · 2025Article
- COPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Empowering rice resilience: elevating starch quality via seed priming amidst bio-molecular challenges during reproductive and maturation phases under drought stress.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Engineering of nicotianamine synthesis enhances cadmium mobility in plants and results in higher seed cadmium concentrations.The Plant journal : for cell and molecular biology · 2025Article
- Barriers and carriers for transition metal homeostasis in plants.Plant communications · 2025Review
- Metabolite Profiling of the Resurrection GrassPlants (Basel, Switzerland) · 2025Article
- The Role of Low-Molecular-Weight Organic Acids in Metal Homeostasis in Plants.International journal of molecular sciences · 2024Review
- Soil and Mineral Nutrients in Plant Health: A Prospective Study of Iron and Phosphorus in the Growth and Development of Plants.Current issues in molecular biology · 2024Review
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Comparative Bioactive Compounds and Mineral Properties of South African and LesothoPlants (Basel, Switzerland) · 2024Article
- A major role of coumarin-dependent ferric iron reduction in strategy I-type iron acquisition in Arabidopsis.The Plant cell · 2024Article
- A CYBDOM protein impacts iron homeostasis and primary root growth under phosphate deficiency in Arabidopsis.Nature communications · 2024Article
- L-DOPA induces iron accumulation in roots of Ipomoea aquatica and Arabidopsis thaliana in a pH-dependent manner.Botanical studies · 2023Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron (Fe) is essential for virtually all living organisms. The identification of the chemical forms of iron (the speciation) circulating in and between cells is crucial to further understand the mechanisms of iron delivery to its final targets. Here we analyzed how iron is transported to the seeds by the chemical identification of iron complexes that are delivered to embryos, followed by the biochemical characterization of the transport of these complexes by the embryo, using the pea (Pisum sativum) as a model species. We have found that iron circulates as ferric complexes with citrate and malate (Fe(III)3Cit2Mal2, Fe(III)3Cit3Mal1, Fe(III)Cit2). Because dicotyledonous plants only transport ferrous iron, we checked whether embryos were capable of reducing iron of these complexes. Indeed, embryos did express a constitutively high ferric reduction activity. Surprisingly, iron(III) reduction is not catalyzed by the expected membrane-bound ferric reductase. Instead, embryos efflux high amounts of ascorbate that chemically reduce iron(III) from citrate-malate complexes. In vitro transport experiments on isolated embryos using radiolabeled (55)Fe demonstrated that this ascorbate-mediated reduction is an obligatory step for the uptake of iron(II). Moreover, the ascorbate efflux activity was also measured in Arabidopsis embryos, suggesting that this new iron transport system may be generic to dicotyledonous plants. Finally, in embryos of the ascorbate-deficient mutants vtc2-4, vtc5-1, and vtc5-2, the reducing activity and the iron concentration were reduced significantly. Taken together, our results identified a new iron transport mechanism in plants that could play a major role to control iron loading in seeds.
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