ArticleScience signaling2013
Deep sequencing and high-resolution imaging reveal compartment-specific localization of Bdnf mRNA in hippocampal neurons.
Article in Science signaling, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- miRNA regulation in brain tissue space: the 3'UTR perspective.RNA (New York, N.Y.) · 2026Review
- Decoding the interactions and functions of non-coding RNA with artificial intelligence.Nature reviews. Molecular cell biology · 2025Review
- Untranslated regions of brain-derived neurotrophic factor mRNA control its translatability and subcellular localization.The Journal of biological chemistry · 2023Article
- Neurotrophin signalling in the human nervous system.Frontiers in molecular neuroscience · 2023Review
- RNA and neuronal function: the importance of post-transcriptional regulation.Oxford open neuroscience · 2022Review
- An increase in VGF expression through a rapid, transcription-independent, autofeedback mechanism improves cognitive function.Translational psychiatry · 2021Article
- Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity.Cell reports · 2021Article
- Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation.The Journal of cell biology · 2020Article
- BDNF Expression in Cortical GABAergic Interneurons.International journal of molecular sciences · 2020Article
- Sonic hedgehog expression in the postnatal brain.Biology open · 2019Article
- Differential regulation of brain-derived neurotrophic factor (BDNF) expression in sensory neuron axons by miRNA-206.FEBS open bio · 2019Article
- Axonal Ensheathment in the Nervous System of Lamprey: Implications for the Evolution of Myelinating Glia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS.Nature communications · 2018Article
- Post-transcriptional Processing of mRNA in Neurons: The Vestiges of the RNA World Drive Transcriptome Diversity.Frontiers in molecular neuroscience · 2018Review
- Neurobiology of local and intercellular BDNF signaling.Pflugers Archiv : European journal of physiology · 2017Review
- Local translation in neuronal compartments: how local is local?EMBO reports · 2017Review
- Is Arc mRNA Unique: A Search for mRNAs That Localize to the Distal Dendrites of Dentate Gyrus Granule Cells Following Neural Activity.Frontiers in molecular neuroscience · 2017Article
- Screening the Molecular Framework Underlying Local Dendritic mRNA Translation.Frontiers in molecular neuroscience · 2017Review
- The Regulation of Synaptic Protein Turnover.The Journal of biological chemistry · 2015Review
- Single-molecule insights into mRNA dynamics in neurons.Trends in cell biology · 2015Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Brain-derived neurotrophic factor (BDNF) is a small protein of the neurotrophin family that regulates various brain functions. Although much is known about how its transcription is regulated, the abundance of endogenous BDNF mRNA and its subcellular localization pattern are matters of debate. We used next-generation sequencing and high-resolution in situ hybridization in the rat hippocampus to reexamine this question. We performed 3' end sequencing on rat hippocampal slices and detected two isoforms of Bdnf containing either a short or a long 3' untranslated region (3'UTR). Most of the Bdnf transcripts contained the short 3'UTR isoform and were present in low amounts relative to other neuronal transcripts. Bdnf mRNA was present in the somatic compartment of rat hippocampal slices or the somata of cultured rat hippocampal neurons but was rarely detected in the dendritic processes. Pharmacological stimulation of hippocampal neurons induced Bdnf expression but did not change the ratio of Bdnf isoform abundance. The findings indicate that endogenous Bdnf mRNA, although weakly abundant, is primarily localized to the somatic compartment of hippocampal neurons. Both Bdnf mRNA isoforms have shorter half-lives compared with other neuronal mRNAs. Furthermore, the findings show that using complementary high-resolution techniques can provide sensitive measures of endogenous transcript abundance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.