ArticleProceedings of the National Academy of Sciences of the United States of America2014
Rewiring yeast sugar transporter preference through modifying a conserved protein motif.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 199 citations in OpenAlex.
- Article
- Biotechnological applications of D-xylose-fermenting autochthonous yeasts in lignocellulosic biorefineries.Biotechnology for biofuels and bioproducts · 2026Review
- Docking-guided and phosphomimetic rational engineering of glucose-insensitive xylose transporters from Trichoderma reesei.Microbial cell factories · 2026Article
- Resurrection and characterization of ancestral xylose transporters enhance the capability of xylose uptake in the mixed sugar co-fermentation of Recombinant Saccharomyces cerevisiae.Bioresources and bioprocessing · 2026Article
- Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose.FEMS yeast research · 2026Article
- High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast.Synthetic and systems biotechnology · 2025Article
- Synergistic effect of transporter and pathway engineering on the key performance indicators of erythritol synthesis by the yeastApplied and environmental microbiology · 2025Article
- Advances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production.Applied microbiology and biotechnology · 2025Review
- Specialization Restricts the Evolutionary Paths Available to Yeast Sugar Transporters.Molecular biology and evolution · 2024Article
- Specialization restricts the evolutionary paths available to yeast sugar transporters.bioRxiv : the preprint server for biology · 2024Article
- A fungal metabolic regulator underlies infectious synergism during Candida albicans-Staphylococcus aureus intra-abdominal co-infection.Nature communications · 2024Article
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters.eLife · 2024Article
- Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium.Nature communications · 2024Article
- Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion.Journal of industrial microbiology & biotechnology · 2024Review
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
- Engineering co-utilization of glucose and xylose for chemical overproduction from lignocellulose.Nature chemical biology · 2023Article
- Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production.Nature communications · 2023Article
- Xylose consumption and ethanol production by Pichia guilliermondii and Candida oleophila in the presence of furans, phenolic compounds, and organic acids commonly produced during the pre-treatment of plant biomass.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Review
15 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Utilization of exogenous sugars found in lignocellulosic biomass hydrolysates, such as xylose, must be improved before yeast can serve as an efficient biofuel and biochemical production platform. In particular, the first step in this process, the molecular transport of xylose into the cell, can serve as a significant flux bottleneck and is highly inhibited by other sugars. Here we demonstrate that sugar transport preference and kinetics can be rewired through the programming of a sequence motif of the general form G-G/F-XXX-G found in the first transmembrane span. By evaluating 46 different heterologously expressed transporters, we find that this motif is conserved among functional transporters and highly enriched in transporters that confer growth on xylose. Through saturation mutagenesis and subsequent rational mutagenesis, four transporter mutants unable to confer growth on glucose but able to sustain growth on xylose were engineered. Specifically, Candida intermedia gxs1 Phe(38)Ile(39)Met(40), Scheffersomyces stipitis rgt2 Phe(38) and Met(40), and Saccharomyces cerevisiae hxt7 Ile(39)Met(40)Met(340) all exhibit this phenotype. In these cases, primary hexose transporters were rewired into xylose transporters. These xylose transporters nevertheless remained inhibited by glucose. Furthermore, in the course of identifying this motif, novel wild-type transporters with superior monosaccharide growth profiles were discovered, namely S. stipitis RGT2 and Debaryomyces hansenii 2D01474. These findings build toward the engineering of efficient pentose utilization in yeast and provide a blueprint for reprogramming transporter properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.