ArticleProceedings of the National Academy of Sciences of the United States of America2014
Initial stages of calcium uptake and mineral deposition in sea urchin embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 154 citations in OpenAlex.
- Possible role of microtubules in vesicular transport of matrix protein during sea urchin larval biomineralization.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Proton channels govern vesicular carbonate chemistry in mineralizing cells of a marine calcifier.Nature communications · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Insights on the intracellular trafficking of calcifying medium in a reef-building coral.Communications biology · 2025Article
- CMTM4 is an adhesion modulator that regulates skeletal patterning and primary mesenchyme cell migration in sea urchin embryos.Developmental biology · 2025Article
- The native glycocalyx ultrastructure in humans and sponges is a self-assembled, lamellar micro- and nanoarray.Communications biology · 2024Article
- Exploring the Potential of Nonclassical Crystallization Pathways to Advance Cementitious Materials.Chemical reviews · 2024Review
- ROCK and the actomyosin network control biomineral growth and morphology during sea urchin skeletogenesis.eLife · 2024Article
- Mg-rich amorphous to Mg-low crystalline CaCOHeliyon · 2023Article
- Rapid grain boundary diffusion in foraminifera tests biases paleotemperature records.Communications earth & environment · 2023Article
- Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF.Materials (Basel, Switzerland) · 2022Article
- Distinct regulatory states control the elongation of individual skeletal rods in the sea urchin embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Defining Local Chemical Conditions in Magnetosomes of Magnetotactic Bacteria.The journal of physical chemistry. B · 2022Article
- Biomineralization: Integrating mechanism and evolutionary history.Science advances · 2022Review
- The Evolution of Biomineralization through the Co-Option of Organic Scaffold Forming Networks.Cells · 2022Review
- A protease-mediated switch regulates the growth of magnetosome organelles inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Ion Pathways in Biomineralization: Perspectives on Uptake, Transport, and Deposition of Calcium, Carbonate, and Phosphate.Journal of the American Chemical Society · 2021Article
- An otopetrin family proton channel promotes cellular acid efflux critical for biomineralization in a marine calcifier.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Liquid-phase ASEM imaging of cellular and structural details in cartilage and bone formed during endochondral ossification: Keap1-deficient osteomalacia.Scientific reports · 2021Article
- Multiple Pathways for Pathological Calcification in the Human Body.Advanced healthcare materials · 2021Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sea urchin larvae have an endoskeleton consisting of two calcitic spicules. We reconstructed various stages of the formation pathway of calcium carbonate from calcium ions in sea water to mineral deposition and integration into the forming spicules. Monitoring calcium uptake with the fluorescent dye calcein shows that calcium ions first penetrate the embryo and later are deposited intracellularly. Surprisingly, calcium carbonate deposits are distributed widely all over the embryo, including in the primary mesenchyme cells and in the surface epithelial cells. Using cryo-SEM, we show that the intracellular calcium carbonate deposits are contained in vesicles of diameter 0.5-1.5 μm. Using the newly developed airSEM, which allows direct correlation between fluorescence and energy dispersive spectroscopy, we confirmed the presence of solid calcium carbonate in the vesicles. This mineral phase appears as aggregates of 20-30-nm nanospheres, consistent with amorphous calcium carbonate. The aggregates finally are introduced into the spicule compartment, where they integrate into the growing spicule.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.