ArticleMolecular genetics and genomics : MGG2014
Identification and profiling of novel and conserved microRNAs during the flower opening process in Prunus mume via deep sequencing.
Article in Molecular genetics and genomics : MGG, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 53 citations in OpenAlex.
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- Integrated transcriptome and small RNA sequencing in revealing miRNA-mediated regulatory network of floral bud break inFrontiers in plant science · 2022Article
- MicroRNAs in Woody Plants.Frontiers in plant science · 2021Review
- Investigation of genes associated with petal variations between diploid and autotetraploid in Chinese cabbage (Brassica rapa L. ssp. pekinensis) by RNA-seq and sRNA-seq.Molecular genetics and genomics : MGG · 2020Article
- Genome-wide discovery and characterization of flower development related long non-coding RNAs in Prunus mume.BMC plant biology · 2019Article
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- Identification and comparative analysis of thePeerJ · 2019Article
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- Characterization of Conserved and Novel microRNAs in Lilium lancifolium Thunb. by High-Throughput Sequencing.Scientific reports · 2018Article
- Genome-Wide Analysis Characterization and Evolution ofFrontiers in genetics · 2018Article
- Genome-wide analysis of miRNAs in Carya cathayensis.BMC plant biology · 2017Article
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- In silico search and biological validation of microRNAs related to drought response in peach and almond.Functional & integrative genomics · 2017Article
- Identification and comparative profiling of miRNAs in herbaceous peony (Paeonia lactiflora Pall.) with red/yellow bicoloured flowers.Scientific reports · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small (20-24 nucleotide) RNAs that are critical regulators of genes involved in diverse plant processes, including development, metabolism, abiotic stress and flowering. Prunus mume is a widely cultivated ornamental plant in East Asia that blooms in early spring, even at temperatures below 0 °C. While miRNAs involved in pistil development have been identified in P. mume, few studies have profiled miRNA expression patterns during flower opening. Here, we used high-throughput sequencing and bioinformatic analysis to identify and profile miRNAs that function during flower opening in P. mume. We identified 47 conserved miRNA sequences belonging to 25 miRNA families from 92 loci in P. mume, along with 33 novel miRNA sequences from 43 loci, including their complementary miRNA* strands. The expression levels of most differentially expressed miRNAs decreased during flower opening, while miR156e-f and miR477b were upregulated at the flowering stage. We predicted 88 target genes for conserved and novel miRNAs using computational analysis and annotated their functions. Seven target genes, encoding squamosa promoter binding protein-like (SPL) and auxin response factor (ARF), scarecrow-like transcription factor (SCL) and APETALA2-like transcription factors (AP2), were verified by 5'-RACE to be the targets of miR156, miR167, miR171 and miR172, respectively. Quantitative real-time PCR validated the expression of the miRNAs and seven target genes. The results help lay the foundation for investigating the roles of miRNAs in the blooming of P. mume.
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