ArticleInfection and immunity2014
Assembly of NADPH oxidase in human neutrophils is modulated by the opacity-associated protein expression State of Neisseria gonorrhoeae.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Neutrophil CEACAMs and the Inflammatory Response toJournal of innate immunity · 2026Review
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- Development and implementation of a Type I-C CRISPR-based programmable repression system formBio · 2024Article
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- The vacuolar anti-Frontiers in immunology · 2024Article
- Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils.Journal of leukocyte biology · 2023Article
- Neisseria gonorrhoeae co-opts C4b-binding protein to enhance complement-independent survival from neutrophils.PLoS pathogens · 2023Article
- Modulation of Oxidative Stress-Induced Senescence during Non-Alcoholic Fatty Liver Disease.Antioxidants (Basel, Switzerland) · 2022Review
- Variable Expression of Opa Proteins by Neisseria gonorrhoeae Influences Bacterial Association and Phagocytic Killing by Human Neutrophils.Journal of bacteriology · 2022Article
- Article
- Phagocytosis and neutrophil extracellular traps.Faculty reviews · 2020Review
- The roles of NADPH oxidase in modulating neutrophil effector responses.Molecular oral microbiology · 2019Review
- Progress Toward a Gonococcal Vaccine: The Way Forward.Frontiers in immunology · 2019Review
- Protocols to Interrogate the Interactions Between Neisseria gonorrhoeae and Primary Human Neutrophils.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Gonococcal Defenses against Antimicrobial Activities of Neutrophils.Trends in microbiology · 2018Review
- Filifactor alocis modulates human neutrophil antimicrobial functional responses.Cellular microbiology · 2018Article
- Pathogenesis of Neisseria gonorrhoeae in the female reproductive tract: neutrophilic host response, sustained infection, and clinical sequelae.Current opinion in hematology · 2018Review
- Neutrophils and Bacterial Immune Evasion.Journal of innate immunity · 2018Review
- Pseudomonas aeruginosa Effector ExoS Inhibits ROS Production in Human Neutrophils.Cell host & microbe · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neisseria gonorrhoeae (the gonococcus, Gc) triggers a potent inflammatory response and recruitment of neutrophils to the site of infection. Gc survives exposure to neutrophils despite these cells' antimicrobial products, such as reactive oxygen species (ROS). ROS production in neutrophils is initiated by NADPH oxidase, which converts oxygen into superoxide. The subunits of NADPH oxidase are spatially separated between granules (gp91(phox)/p22(phox)) and the cytoplasm (p47(phox), p67(phox), and p40(phox)). Activation of neutrophils promotes the coassembly of NADPH oxidase subunits at phagosome and/or plasma membranes. While Gc-expressing opacity-associated (Opa) proteins can induce neutrophils to produce ROS, Opa-negative (Opa-) Gc does not stimulate neutrophil ROS production. Using constitutively Opa- and OpaD-positive (OpaD+) Gc bacteria in strain FA1090, we now show that the difference in ROS production levels in primary human neutrophils between these backgrounds can be attributed to differential assembly of NADPH oxidase. Neutrophils infected with Opa- Gc showed limited translocation of NADPH oxidase cytoplasmic subunits to cellular membranes, including the bacterial phagosome. In contrast, these subunits rapidly translocated to neutrophil membranes following infection with OpaD+ Gc. gp91(phox) and p22(phox) were recruited to Gc phagosomes regardless of bacterial Opa expression. These results suggest that Opa- Gc interferes with the recruitment of neutrophil NADPH oxidase cytoplasmic subunits to membranes, in particular, the p47(phox) "organizing" subunit, to prevent assembly of the holoenzyme, resulting in an absence of the oxidative burst.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.