ArticleHuman vaccines & immunotherapeutics2014
Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix(®) and RotaTeq(®) vaccine strains in stool samples.
Article in Human vaccines & immunotherapeutics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Abundance of single filamentous bacteria, and expression of differentiated Th17 cells, their signature cytokine IL-17 A, and retinoic acid receptor are predictive of poor rotavirus vaccine take.Virology journal · 2025Article
- Article
- Expression of salivary hepcidin and its inducer, interleukin 6 as well as type I interferons are significantly elevated in infants with poor oral rotavirus vaccine take in South Africa.Frontiers in immunology · 2025Article
- Identification of Rotavirus Genotypes in Children under Five Years in the United Arab Emirates Using Nanopore Sequencing Technology.Journal of medical virology · 2024Article
- Correlates of Rotavirus Vaccine Shedding and Seroconversion in a US Cohort of Healthy Infants.The Journal of infectious diseases · 2024Article
- Development of a TaqMan polymerase chain reaction detection method for the precise identification and quantification of an attenuatedFrontiers in veterinary science · 2024Article
- Nonsecretor Phenotype Is Associated With Less Risk of Rotavirus-Associated Acute Gastroenteritis in a Vaccinated Nicaraguan Birth Cohort.The Journal of infectious diseases · 2023Article
- Similarities in rotavirus vaccine viral shedding and immune responses in pairs of twins.Fujita medical journal · 2023Article
- Genotype Diversity of Enteric Viruses in Wastewater Amid the COVID-19 Pandemic.Food and environmental virology · 2023Article
- Rotavirus Strain Trends in United States, 2009-2016: Results from the National Rotavirus Strain Surveillance System (NRSSS).Viruses · 2022Review
- Article
- Detection of Rotavirus Strains in Freshwater Clams in Japan.Food and environmental virology · 2022Article
- Case Report: Rotavirus Vaccination and Severe Combined Immunodeficiency in Japan.Frontiers in immunology · 2022Article
- Temporal variations of human and animalFrontiers in microbiology · 2022Article
- Development of a Real-Time Reverse Transcription-PCR Assay To Detect and Quantify Group A Rotavirus Equine-Like G3 Strains.Journal of clinical microbiology · 2021Article
- Detection and Clinical Implications of Monovalent Rotavirus Vaccine-Derived Virus Strains in Children with Gastroenteritis in Alberta, Canada.Journal of clinical microbiology · 2021Article
- Detection of Rotavirus Vaccine Strains in Oysters and Sewage and Their Relationship with the Gastroenteritis Epidemic.Applied and environmental microbiology · 2021Article
- Group A Rotavirus Detection and Genotype Distribution before and after Introduction of a National Immunisation Programme in Ireland: 2015-2019.Pathogens (Basel, Switzerland) · 2020Article
- Nonsecretor Histo-blood Group Antigen Phenotype Is Associated With Reduced Risk of Clinical Rotavirus Vaccine Failure in Malawian Infants.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group A rotaviruses (RVA) are the leading cause of severe diarrhea in young children worldwide. Two live-attenuated RVA vaccines, Rotarix(®) and RotaTeq(®) are recommended by World Health Organization (WHO) for routine immunization of all infants. Rotarix(®) and RotaTeq(®) vaccines have substantially reduced RVA associated mortality but occasionally have been associated with acute gastroenteritis (AGE) cases identified in vaccinees and their contacts. High-throughput assays are needed to monitor the prevalence of vaccine strains in AGE cases and emergence of new vaccine-derived strains following RVA vaccine introduction. In this study, we have developed quantitative real-time RT-PCR (qRT-PCR) assays for detection of Rotarix(®) and RotaTeq(®) vaccine components in stool samples. Real-time RT-PCR assays were designed for vaccine specific targets in the genomes of Rotarix(®) (NSP2, VP4) and RotaTeq(®) (VP6, VP3-WC3, VP3-human) and validated on sequence confirmed stool samples containing vaccine strains, wild-type RVA strains, and RVA-negative stools. For quantification, standard curves were generated using dsRNA transcripts derived from RVA gene segments. Rotarix(®) NSP2 and VP4 qRT-PCR assays exhibited 92-100% sensitivity, 99-100% specificity, 94-105% efficiency, and a limit of detection of 2-3 copies per reaction. RotaTeq(®) VP6, VP3-WC3, and VP3-human qRT-PCR assays displayed 100% sensitivity, 94-100% specificity, 91-102% efficiency and limits of detection of 1 copy, 2 copies, and 140 copies, respectively. These assays permit rapid identification of Rotarix(®) and RotaTeq(®) vaccine components in stool samples from clinical and surveillance studies and will be helpful in determining the frequency of vaccine strain-associated AGE.
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