ArticleJournal of the American Chemical Society2014
Lipoprotein activators stimulate Escherichia coli penicillin-binding proteins by different mechanisms.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Streptococcus pneumoniae S protein activates PBP1a to regulate peptidoglycan remodelling and cell division.Nature microbiology · 2026Article
- The hit-and-run of cell wall synthesis: LpoB transiently binds and activates PBP1b through a conserved allosteric switch.Nature communications · 2025Article
- Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children.BMC microbiology · 2025Article
- Tazobactam selects for multidrug resistance.npj antimicrobials and resistance · 2025Article
- Biotechnological potential of silver nanoparticles synthesized by green method to control phytopathogenic bacteria: contributions from a proteomic analysis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Biochemical and crystallographic studies of L,D-transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate.Communications biology · 2024Article
- Article
- Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Escherichia coli has robust regulatory mechanisms against elevated peptidoglycan cleavage by lytic transglycosylases.The Journal of biological chemistry · 2023Article
- Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate.Cell surface (Amsterdam, Netherlands) · 2022Article
- A Defect in Lipoprotein Modification by Lgt Leads to Abnormal Morphology and Cell Death in Escherichia coli That Is Independent of Major Lipoprotein Lpp.Journal of bacteriology · 2022Article
- Metabolic Processing of Selenium-Based Bioisosteres ofBiochemistry · 2022Article
- The Bacterial Cell Wall: From Lipid II Flipping to Polymerization.Chemical reviews · 2022Review
- Lytic transglycosylase MltG cleaves in nascent peptidoglycan and produces short glycan strands.Cell surface (Amsterdam, Netherlands) · 2021Article
- The LpoA activator is required to stimulate the peptidoglycan polymerase activity of its cognate cell wall synthase PBP1a.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.Chemical reviews · 2021Review
- Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins.eLife · 2021Article
- Peptidoglycan: Structure, Synthesis, and Regulation.EcoSal Plus · 2021Article
- Regulation of peptidoglycan synthesis and remodelling.Nature reviews. Microbiology · 2020Review
- Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa.Structure (London, England : 1993) · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In Escherichia coli , the bifunctional penicillin-binding proteins (PBPs), PBP1A and PBP1B, play critical roles in the final stage of peptidoglycan (PG) biosynthesis. These synthetic enzymes each possess a PG glycosyltransferase (PGT) domain and a transpeptidase (TP) domain. Recent genetic experiments have shown that PBP1A and PBP1B each require an outer membrane lipoprotein, LpoA and LpoB, respectively, to function properly in vivo. Here, we use complementary assays to show that LpoA and LpoB each increase the PGT and TP activities of their cognate PBPs, albeit by different mechanisms. LpoA directly increases the rate of the PBP1A TP reaction, which also results in enhanced PGT activity; in contrast, LpoB directly affects PGT domain activity, resulting in enhanced TP activity. These studies demonstrate bidirectional coupling of PGT and TP domain function. Additionally, the transpeptidation assay described here can be applied to study other activators or inhibitors of the TP domain of PBPs, which are validated drug targets.
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Registered trials
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