ArticleStem cells and development2014
Three-dimensional spheroid-cultured mesenchymal stem cells devoid of embolism attenuate brain stroke injury after intra-arterial injection.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Intra-Arterial Administration of Stem Cells and Exosomes for Central Nervous System Disease.International journal of molecular sciences · 2025Review
- Alternating 2D and 3D culture reduces cell size and extends the lifespan of placenta-derived mesenchymal stem cells.Frontiers in bioengineering and biotechnology · 2025Article
- Extracellular vesicles from human adipose-derived stem cell spheroids: Characterization and therapeutic implications in diabetic wound healing.Materials today. Bio · 2024Article
- Scaffold-free 3D culture systems for stem cell-based tissue regeneration.APL bioengineering · 2024Review
- Trans-Arterial Stem Cell Injection (TASI): The Role of Interventional Radiology in Regenerative Medicine.Journal of clinical medicine · 2024Review
- Secretome as a Tool to Treat Neurological Conditions: Are We Ready?International journal of molecular sciences · 2023Review
- Multinuclear MRI Reveals Early Efficacy of Stem Cell Therapy in Stroke.Translational stroke research · 2023Article
- Improving vasculoprotective effects of MSCs in coronary microvessels - benefits of 3D culture, sub-populations and heparin.Frontiers in immunology · 2023Article
- Article
- Brain Homogenate of a Rat Model of Alzheimer's Disease Modifies the Secretome of 3D Cultured Periodontal Ligament Stem Cells: A Potential Neuroregenerative Therapy.Iranian journal of pharmaceutical research : IJPR · 2022Article
- Mesenchymal stem cell aggregation mediated by integrin α4/VCAM-1 after intrathecal transplantation in MCAO rats.Stem cell research & therapy · 2022Article
- Three-dimensional spheroids of dedifferentiated fat cells enhance bone regeneration.Regenerative therapy · 2021Article
- 3D spheroids of human placenta-derived mesenchymal stem cells attenuate spinal cord injury in mice.Cell death & disease · 2021Article
- Three-dimensional cultured mesenchymal stem cells enhance repair of ischemic stroke through inhibition of microglia.Stem cell research & therapy · 2021Article
- Aggregation of Human Mesenchymal Stromal Cells Eliminates Their Ability to Suppress Human T Cells.Frontiers in immunology · 2020Article
- A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids.PloS one · 2020Article
- Aggregation of human mesenchymal stem cells enhances survival and efficacy in stroke treatment.Cytotherapy · 2019Article
- Three-Dimensional Spheroid Formation of Cryopreserved Human Dental Follicle-Derived Stem Cells Enhances Pluripotency and Osteogenic Induction Properties.Tissue engineering and regenerative medicine · 2019Article
- Immunomodulatory Functions of Mesenchymal Stem Cells in Tissue Engineering.Stem cells international · 2019Review
- Three-Dimensional Spheroid Culture Increases Exosome Secretion from Mesenchymal Stem Cells.Tissue engineering and regenerative medicine · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The therapeutic effect of mesenchymal stem cells (MSCs) in tissue repair/regeneration is substantially dampened by the loss of primitive properties and poor engraftment to target organs. In this study, the multipotency and cell sizes of human MSCs, which had been expanded in monolayer culture for several passages, were dramatically restored after an episode of three-dimensional (3D) spheroid culture. Unlike MSCs derived from monolayer, which caused embolism and blindness, MSCs derived from 3D spheroids did not cause vascular obstructions, after intra-carotid artery infusion in rats. Importantly, intra-carotid infusion of 1 million 3D spheroid MSCs in rats 24 h after middle cerebral artery occlusion and reperfusion resulted in engraftment of the cells into the lesion and significant (over 70%) reduction of infarct size along with restoration of neurologic function. Moreover, the enhanced effect of spheroid MSCs was coincided with significantly increased differentiation of the MSCs into neurons and markedly increased number of endogenous glial fibrillary acidic protein-positive neural progenitors in the peri-infarct boundary zone. However, the similarly administered monolayer MSCs resulted in a modest functional improvement. Our results suggest that 3D MSCs, in combination with intra-carotid delivery, may represent a novel therapeutic approach of MSCs for stroke.
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