ArticleACS nano2014
Three-dimensional hydrodynamic focusing method for polyplex synthesis.
Article in ACS nano, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- High-Efficiency l‑PEI-Based Transfection of ARPE-19 Cells Using a Multiparametric Approach and Automated Polyplex Formation with a 3D-Printed Microfluidic System.Chem & bio engineering · 2025Article
- Optimizing Microfluidic Channel Design with Tilted Rectangular Baffles for Enhanced mRNA-Lipid Nanoparticle Preparation.ACS biomaterials science & engineering · 2025Article
- Development of novel lipoplex formulation methodologies to improve large-scale transient transfection for lentiviral vector manufacture.Molecular therapy. Methods & clinical development · 2024Article
- Flash nanoprecipitation assisted self-assembly of ionizable lipid nanoparticles for nucleic acid delivery.Nanoscale · 2024Article
- Synthesis of Nanogels: Current Trends and Future Outlook.Gels (Basel, Switzerland) · 2021Review
- Heat-shrinking DNA nanoparticles for in vivo gene delivery.Gene therapy · 2020Article
- Acoustofluidic Synthesis of Particulate Nanomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Article
- The role of assembly parameters on polyplex poly(beta-amino ester) nanoparticle transfections.Biotechnology and bioengineering · 2019Article
- Article
- Biomaterials Meet Microfluidics: From Synthesis Technologies to Biological Applications.Micromachines · 2017Review
- Microfluidic Hydrodynamic Focusing for Synthesis of Nanomaterials.Nano today · 2016Article
- Can microfluidics address biomanufacturing challenges in drug/gene/cell therapies?Regenerative biomaterials · 2016Review
- Shape-controlled synthesis of hybrid nanomaterials via three-dimensional hydrodynamic focusing.ACS nano · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Successful intracellular delivery of nucleic acid therapeutics relies on multiaspect optimization, one of which is formulation. While there has been ample innovation on chemical design of polymeric gene carriers, the same cannot be said for physical processing of polymer-DNA nanocomplexes (polyplexes). Conventional synthesis of polyplexes by bulk mixing depends on the operators' experience. The poorly controlled bulk mixing process may also lead to batch-to-batch variation and consequent irreproducibility. Here, we synthesize polyplexes by using a three-dimensional hydrodynamic focusing (3D-HF) technique in a single-layered, planar microfluidic device. Without any additional chemical treatment or postprocessing, the polyplexes prepared by the 3D-HF method show smaller size, slower aggregation rate, and higher transfection efficiency, while exhibiting reduced cytotoxicity compared to the ones synthesized by conventional bulk mixing. In addition, by introducing external acoustic perturbation, mixing can be further enhanced, leading to even smaller nanocomplexes. The 3D-HF method provides a simple and reproducible process for synthesizing high-quality polyplexes, addressing a critical barrier in the eventual translation of nucleic acid therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.