ArticlePloS one2013
Differentiation and migration of bone marrow mesenchymal stem cells transplanted through the spleen in rats with portal hypertension.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
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- Tracking of Stem Cells in Chronic Liver Diseases: Current Trends and Developments.Stem cell reviews and reports · 2024Review
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- Genetic modification by overexpression of target gene in mesenchymal stromal cell for treating liver diseases.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Stem cell-derived extracellular vesicles: role in oncogenic processes, bioengineering potential, and technical challenges.Stem cell research & therapy · 2019Review
- Secretome of Mesenchymal Stem Cells and Its Potential Protective Effects on Brain Pathologies.Molecular neurobiology · 2019Review
- A Novel Role of VEGFC in Cerebral Ischemia With Lung Injury.Frontiers in neuroscience · 2019Article
- Effect of Human Wnt10b Transgene Overexpression on Peri-Implant Osteogenesis in Ovariectomized Rats.Human gene therapy · 2018Article
- Neural Stem Cell Transplantation Promotes Functional Recovery from Traumatic Brain Injury via Brain Derived Neurotrophic Factor-Mediated Neuroplasticity.Molecular neurobiology · 2018Article
- The potassium channel KCa3.1 constitutes a pharmacological target for astrogliosis associated with ischemia stroke.Journal of neuroinflammation · 2017Article
- Bone Marrow Mesenchymal Stem-Cell Transplantation Promotes Functional Improvement Associated with CNTF-STAT3 Activation after Hemi-Sectioned Spinal Cord Injury in Tree Shrews.Frontiers in cellular neuroscience · 2017Article
- MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection.Scientific reports · 2016Article
- P2Y1, P2Y2, and TRPV1 Receptors Are Increased in Diarrhea-Predominant Irritable Bowel Syndrome and P2Y2 Correlates with Abdominal Pain.Digestive diseases and sciences · 2016Article
- Noninvasive in-vivo tracing and imaging of transplanted stem cells for liver regeneration.Stem cell research & therapy · 2016Review
- Association of Ulcerative Colitis with FUT2 and FUT3 Polymorphisms in Patients from Southeast China.PloS one · 2016Article
- Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure.Advanced pharmaceutical bulletin · 2015Review
- Adenoviral vector-mediated overexpression of osteoprotegerin accelerates osteointegration of titanium implants in ovariectomized rats.Gene therapy · 2015Article
- Using reconfigurable microfluidics to study the role of HGF in autocrine and paracrine signaling of hepatocytes.Integrative biology : quantitative biosciences from nano to macro · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe goals of this paper were to evaluate the differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro, and to determine whether stem cells can migrate and plant into the liver with portal hypertension accompanied by the end-stage of liver disease.
methodsBMSCs were isolated from rats and amplified with hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4). The expression of alpha-fetoprotein (AFP), cytokeratin 18 (CK-18), and albumin (ALB) was detected by immunofluorescence in induced cells. Rats were randomly divided into experimental (with common bile duct ligation) and control groups. After injection of fluorescence labeled cells, cell distribution was observed under a fluorescence microscope. The integrated optical density (IOD) and cell distribution scores were evaluated using Image-Pro Plus 6.0 software. The portal pressure was measured before the rats were killed.
resultsAfter being induced with HGF and FGF-4, the Golgi apparatus, endoplasmic reticulum, ribosomes, and mitochondria all significantly increased in the fifth generation cells. Immunofluorescent analysis showed that the induced cells expressed AFP, CK-18, and ALB. BMSCs were stained by CM-Dil, and the labeling rate was as high as 95.5%. The portal pressure in experimental group was much higher than that of the control group (18.04±2.35 vs. 9.75±1.40 cm H2O p<0.01). The IOD of transplanted cells in the experimental group was also significantly higher than that of the control group (11.30±2.09×10(5) vs. 2.93±0.88×10(5), p<0.01). In addition, the cell distribution score in the experimental group was lower than that of the control group (1.99±0.36 vs. 2.36±0.27, P<0.05).
conclusionsThe combination of HGF and FGF-4 induces the differentiation of BMSCs into hepatocyte-like cells, which express AFP, CK-18, and ALB. In addition, the recruitment of BMSCs (after transplantation in the spleen) was improved in rats with portal hypertension.
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