ArticlePloS one2013
Identification and characterization of a PutAMT1;1 gene from Puccinellia tenuiflora.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Genome-wide analysis of the ammonium transporters gene family in Phaseolus vulgaris and its roles in response to drought and salinity stress.Scientific reports · 2025Article
- Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses.Functional & integrative genomics · 2019Article
- The role of ammonium transporter (AMT) against salt stress in plants.Plant signaling & behavior · 2019Article
- The ctenidium of the giant clam, Tridacna squamosa, expresses an ammonium transporter 1 that displays light-suppressed gene and protein expression and may be involved in ammonia excretion.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Host-derived viral transporter protein for nitrogen uptake in infected marine phytoplankton.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea.Genetics and molecular biology · 2017Article
- Overexpression of AtOxR gene improves abiotic stresses tolerance and vitamin C content in Arabidopsis thaliana.BMC biotechnology · 2016Article
- Abiotic Stresses Downregulate Key Genes Involved in Nitrogen Uptake and Assimilation in Brassica juncea L.PloS one · 2015Article
- Transcriptional responses of a bicarbonate-tolerant monocot, Puccinellia tenuiflora, and a related bicarbonate-sensitive species, Poa annua, to NaHCO3 stress.International journal of molecular sciences · 2014Article
- An efficient method for stable protein targeting in grasses (Poaceae): a case study in Puccinellia tenuiflora.BMC biotechnology · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrogen is one of the most important limiting factors for plant growth. However, as ammonium is readily converted into ammonia (NH3) when soil pH rises above 8.0, this activity depletes the availability of ammonium (NH4(+)) in alkaline soils, consequently preventing the growth of most plant species. The perennial wild grass Puccinellia tenuiflora is one of a few plants able to grow in soils with extremely high salt and alkaline pH (>9.0) levels. Here, we assessed how this species responds to ammonium under such conditions by isolating and analyzing the functions of a putative ammonium transporter (PutAMT1;1). PutAMT1;1 is the first member of the AMT1 (ammonium transporter) family that has been identified in P. tenuiflora. This gene (1) functionally complemented a yeast mutant deficient in ammonium uptake (2), is preferentially expressed in the anther of P. tenuiflora, and (3) is significantly upregulated by ammonium ions in both the shoot and roots. The PutAMT1;1 protein is localized in the plasma membrane and around the nuclear periphery in yeast cells and P. tenuiflora suspension cells. Immunoelectron microscopy analysis also indicated that PutAMT1;1 is localized in the endomembrane. The overexpression of PutAMT1;1 in A. thaliana enhanced plant growth, and increased plant susceptibility to toxic methylammonium (MeA). Here, we confirmed that PutAMT1;1 is an ammonium-inducible ammonium transporter in P. tenuiflora. On the basis of the results of PutAMT1;1 overexpression in A. thaliana, this gene might be useful for improving the root to shoot mobilization of MeA (or NH4(+)).
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