ArticlePloS one2013
A soluble fucose-specific lectin from Aspergillus fumigatus conidia--structure, specificity and possible role in fungal pathogenicity.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 104 citations in OpenAlex.
- Dynamic Combinatorial Chemistry Generates Adaptative Libraries of Glyco-Dyn[n]Arenes That can Be Templated to Produce Anti-Adhesive Glycoconjugates Targeting Pseudomonas aeruginosa.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Requirement for Fucosyltransferase 2 in Allergic Airway Hyperreactivity and Mucus Obstruction.American journal of respiratory cell and molecular biology · 2025Article
- A fucose-binding superlectin from Enterobacter cloacae with high Lewis and ABO blood group antigen specificity.The Journal of biological chemistry · 2025Article
- In-depth comparative transcriptome analysis of Purpureocillium sp. CB1 under cadmium stress.Applied microbiology and biotechnology · 2023Article
- Comparative proteome analysis identifies species-specific signature proteins in Aspergillus pathogens.Applied microbiology and biotechnology · 2023Article
- Multivalent Fucosides Targeting β-Propeller Lectins from Lung Pathogens with Promising Anti-Adhesive Properties.ACS chemical biology · 2022Article
- Cytokines help suggest aplastic anemia with pulmonary bacterial or co-fungal infection.Scientific reports · 2022Article
- Multivalent glycocyclopeptides: conjugation methods and biological applications.Chemical Society reviews · 2022Review
- Novel Treatment Approach for Aspergilloses by Targeting Germination.Journal of fungi (Basel, Switzerland) · 2022Review
- Monoclonal Antibodies and Invasive Aspergillosis: Diagnostic and Therapeutic Perspectives.International journal of molecular sciences · 2022Review
- Druggable Allosteric Sites in β-Propeller Lectins.Angewandte Chemie (International ed. in English) · 2022Article
- Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.Membranes · 2021Review
- Review
- Biochemical and structural studies of target lectin SapL1 from the emerging opportunistic microfungus Scedosporium apiospermum.Scientific reports · 2021Article
- Aspergillus fumigatus biofilms: Toward understanding how growth as a multicellular network increases antifungal resistance and disease progression.PLoS pathogens · 2021Review
- A Comprehensive Phylogenetic and Bioinformatics Survey of Lectins in the Fungal Kingdom.Journal of fungi (Basel, Switzerland) · 2021Article
- Glycan array analysis of Pholiota squarrosa lectin and other fucose-oriented lectins.Glycobiology · 2021Article
- Development of 48-condition buffer screen for protein stability assessment.European biophysics journal : EBJ · 2021Article
- VirulenceFrontiers in molecular biosciences · 2021Review
- Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray.Frontiers in oncology · 2021Article
Corrections and comments
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Authors and funding
12 authors at 9 institutions in 4 countries.
Funding
Abstract
Aspergillus fumigatus is an important allergen and opportunistic pathogen. Similarly to many other pathogens, it is able to produce lectins that may be involved in the host-pathogen interaction. We focused on the lectin AFL, which was prepared in recombinant form and characterized. Its binding properties were studied using hemagglutination and glycan array analysis. We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including α1-6 linked core-fucose, which is an important marker for cancerogenesis. Other biologically relevant saccharides such as sialic acid, d-mannose or d-galactose were not bound. Blood group epitopes of the ABH and Lewis systems were recognized, Le(Y) being the preferred ligand among others. To provide a correlation between the observed functional characteristics and structural basis, AFL was crystallized in a complex with methyl-α,L-selenofucoside and its structure was solved using the SAD method. Six binding sites, each with different compositions, were identified per monomer and significant differences from the homologous AAL lectin were found. Structure-derived peptides were utilized to prepare anti-AFL polyclonal antibodies, which suggested the presence of AFL on the Aspergillus' conidia, confirming its expression in vivo. Stimulation of human bronchial cells by AFL led to IL-8 production in a dose-dependent manner. AFL thus probably contributes to the inflammatory response observed upon the exposure of a patient to A. fumigatus. The combination of affinity to human epithelial epitopes, production by conidia and pro-inflammatory activity is remarkable and shows that AFL might be an important virulence factor involved in an early stage of A. fumigatus infection.
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