ArticlePloS one2013
Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Targeted IL-27-based gene therapy in preventing SARS-CoV-2 entry.Molecular biology reports · 2026Article
- Highly frequent undesired insertional mutagenesis during Drosophila genome editing.PLoS genetics · 2026Article
- Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.Scientific reports · 2025Article
- Enhancing Agrobacterium-mediated soybean transformation efficiency with an auxiliary solution.Crop health · 2024Article
- A Humanized CB1R Yeast Biosensor Enables Facile Screening of Cannabinoid Compounds.International journal of molecular sciences · 2024Article
- Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor.Microbial cell factories · 2024Article
- A simple, rapid fluorescent reporter-based method for detection of ectopic cre recombinase expression in presumed retinal cell type-targeted mouse lines.Experimental eye research · 2023Article
- Human CD79bFrontiers in immunology · 2023Article
- A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination.Nature communications · 2022Article
- Endometriosis in the Mouse: Challenges and Progress Toward a 'Best Fit' Murine Model.Frontiers in physiology · 2021Review
- A transgenic pig model expressing a CMV-ZsGreen1 reporter across an extensive array of tissues.Journal of biomedical research · 2020Article
- Engineering G protein-coupled receptor signalling in yeast for biotechnological and medical purposes.FEMS yeast research · 2020Review
- A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression.Scientific reports · 2019Article
- A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA.Applied and environmental microbiology · 2018Article
- A human iPSC line capable of differentiating into functional macrophages expressing ZsGreen: a tool for the study andPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity.Scientific reports · 2016Article
- Critical Role for CD103(+)/CD141(+) Dendritic Cells Bearing CCR7 for Tumor Antigen Trafficking and Priming of T Cell Immunity in Melanoma.Cancer cell · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-protein-coupled receptors (GPCRs) are currently the most important pharmaceutical targets for drug discovery because they regulate a wide variety of physiological processes. Consequently, simple and convenient detection systems for ligands that regulate the function of GPCR have attracted attention as powerful tools for new drug development. We previously developed a yeast-based fluorescence reporter ligand detection system using flow cytometry. However, using this conventional detection system, fluorescence from a cell expressing GFP and responding to a ligand is weak, making detection of these cells by fluorescence microscopy difficult. We here report improvements to the conventional yeast fluorescence reporter assay system resulting in the development of a new highly-sensitive fluorescence reporter assay system with extremely bright fluorescence and high signal-to-noise (S/N) ratio. This new system allowed the easy detection of GPCR signaling in yeast using fluorescence microscopy. Somatostatin receptor and neurotensin receptor (implicated in Alzheimer's disease and Parkinson's disease, respectively) were chosen as human GPCR(s). The facile detection of binding to these receptors by cognate peptide ligands was demonstrated. In addition, we established a highly sensitive ligand detection system using yeast cell surface display technology that is applicable to peptide screening, and demonstrate that the display of various peptide analogs of neurotensin can activate signaling through the neurotensin receptor in yeast cells. Our system could be useful for identifying lead peptides with agonistic activity towards targeted human GPCR(s).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.