ArticlePloS one2013
Proteomic analysis in nitrogen-deprived Isochrysis galbana during lipid accumulation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Nutrient Deprivation Coupled with High Light Exposure for Bioactive Chrysolaminarin Production in the Marine MicroalgaMarine drugs · 2022Article
- De Novo Transcriptome of the FlagellateBiology · 2022Article
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- Viral growth factor- and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection.PLoS pathogens · 2021Article
- Improvement of Chemical Composition ofMolecules (Basel, Switzerland) · 2020Article
- The Puzzling Conservation and Diversification of Lipid Droplets from Bacteria to Eukaryotes.Results and problems in cell differentiation · 2020Article
- Expression profiling of genes coding for abundant proteins in the alkenone body of marine haptophyte alga Tisochrysis lutea.BMC microbiology · 2019Article
- Increased urea availability promotes adjustments in C/N metabolism and lipid content without impacting growth in Chlamydomonas reinhardtii.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Evidence for PII with NAGK interaction that regulates Arg synthesis in the microalga Myrmecia incisa in response to nitrogen starvation.Scientific reports · 2017Article
- Membrane Proteomic Insights into the Physiology and Taxonomy of an Oleaginous Green Microalga.Plant physiology · 2017Article
- Proteomics analysis of high lipid-producing strain Mucor circinelloides WJ11: an explanation for the mechanism of lipid accumulation at the proteomic level.Microbial cell factories · 2016Article
- Proteomic analysis of lipid body from the alkenone-producing marine haptophyte alga Tisochrysis lutea.Proteomics · 2015Article
- Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii.Biotechnology for biofuels · 2015Article
- Diversity and regulation of ATP sulfurylase in photosynthetic organisms.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differentially co-expressed proteins in N-deprived and N-enriched I. galbana were comparatively analyzed by using two dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight-mass spectrometry (MALDI-TOF/TOF-MS) with the aim of better understanding lipid metabolism in this oleaginous microalga. Forty-five of the 900 protein spots showed dramatic changes in N-deprived I. galbana compared with the N-enriched cells. Of these, 36 protein spots were analyzed and 27 proteins were successfully identified. The identified proteins were classified into seven groups by their molecular functions, including the proteins related to energy production and transformation, substance metabolism, signal transduction, molecular chaperone, transcription and translation, immune defense and cytoskeleton. These altered proteins slowed cell growth and photosynthesis of I. galbana directly or indirectly, but at the same time increased lipid accumulation. Eight key enzymes involved in lipid metabolism via different pathways were identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate kinase (PGK), enolase, aspartate aminotransferase (AST), fumarate hydratase (FH), citrate synthase (CS), O-acetyl-serine lyase (OAS-L) and ATP sulfurylase (ATPS). The results suggested that the glycolytic pathway and citrate transport system might be the main routes for lipid anabolism in N-deprived I. galbana, and that the tricarboxylic acid (TCA) cycle, glyoxylate cycle and sulfur assimilation system might be the major pathways involved in lipid catabolism.
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