ArticlePloS one2013
An improved detection and quantification method for the coral pathogen Vibrio coralliilyticus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- Quorum sensing regulates virulence factors in the coral pathogenApplied and environmental microbiology · 2025Article
- Seasonal dynamics and environmental drivers of tissue and mucus microbiomes in the staghorn coralPeerJ · 2024Article
- A deep-sea bacterium related to coastal marine pathogens.Environmental microbiology · 2021Article
- Bacteriophages against Vibrio coralliilyticus and Vibrio tubiashii: Isolation, Characterization, and Remediation of Larval Oyster Mortalities.Applied and environmental microbiology · 2021Article
- Disease Diagnostics and Potential Coinfections byFrontiers in microbiology · 2020Article
- The Probiotic Bacterium Phaeobacter inhibens Downregulates Virulence Factor Transcription in the Shellfish Pathogen Vibrio coralliilyticus byApplied and environmental microbiology · 2019Article
- Isolation and Pathogenic Characterization ofFrontiers in microbiology · 2019Article
- Environmental Controls of Oyster-Pathogenic Vibrio spp. in Oregon Estuaries and a Shellfish Hatchery.Applied and environmental microbiology · 2018Article
- Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy).Microbial ecology · 2018Article
- Quantitative Detection of Active Vibrios Associated with White Plague Disease inFrontiers in microbiology · 2017Article
- New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects.Frontiers in microbiology · 2017Review
- Article
- First Report ofFrontiers in microbiology · 2016Article
- Draft Genome Sequence of the Marine Pathogen Vibrio coralliilyticus RE22.Genome announcements · 2015Article
- Mortalities of Eastern and Pacific oyster Larvae caused by the pathogens Vibrio coralliilyticus and Vibrio tubiashii.Applied and environmental microbiology · 2015Article
- Complete Genome Sequence for the Shellfish Pathogen Vibrio coralliilyticus RE98 Isolated from a Shellfish Hatchery.Genome announcements · 2014Article
- Complete Genome Sequence of the Larval Shellfish Pathogen Vibrio tubiashii Type Strain ATCC 19109.Genome announcements · 2014Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA- and RNA-based PCR and reverse-transcription real-time PCR assays were developed for diagnostic detection of the vcpA zinc-metalloprotease implicated in the virulence of the coral pathogen Vibrio coralliilyticus. Both PCR methods were highly specific for V. coralliilyticus and failed to amplify strains of closely-related Vibrio species. The assays correctly detected all globally occurring V. coralliilyticus isolates including a newly-described isolate [TAV24] infecting gorgonians in the Mediterranean Sea and highlighted those isolates that had been potentially misidentified, in particular V. tubiashii strains ATCC 19105 and RE22, historically described as important oyster pathogens. The real-time assay is sensitive, detecting 10 gene copies and the relationships between gene copy number and cycle threshold (C T ) were highly linear (R(2)≥ 99.7). The real-time assay was also not affected by interference from non-target DNA. These assays are useful for rapid detection of V. coralliilyticus and monitoring of virulence levels in environmental samples, allowing for implementation of timely management steps to limit and possibly prevent losses due to V. coralliilyticus infection, as well as furthering investigations of factors affecting pathogenesis of this important marine pathogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.