ArticlePloS one2013
Interleukin-8 responses of multi-layer gingival epithelia to subgingival biofilms: role of the "red complex" species.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Effects of initial periodontal treatment on heat shock protein 90 and Interleukin-1β levels in gingival crevicular fluid of stage III periodontitis patients.BMC oral health · 2025Article
- Advances in modeling periodontal host-microbe interactions: insights from organotypic and organ-on-chip systems.Lab on a chip · 2025Review
- Cytokine release and NLRP3 inflammasome activation induced by low-abundance oral bacterial biofilms.Journal of oral microbiology · 2025Article
- Biofabrication Strategies for Oral Soft Tissue Regeneration.Advanced healthcare materials · 2024Review
- Microfluidic organ-on-chip systems for periodontal research: advances and future directions.Frontiers in bioengineering and biotechnology · 2024Review
- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
- Lactobacilli Attenuate the Effect ofFrontiers in microbiology · 2022Article
- Review
- Validation and verification of predictive salivary biomarkers for oral health.Scientific reports · 2021Article
- Immunomodulatory streptococci that inhibit CXCL8 secretion and NFκB activation are common members of the oral microbiota.Journal of medical microbiology · 2021Article
- Gingival Crevicular Fluid Cytokines in Moderate and Deep Sites of Stage III Periodontitis Patients in Different Rates of Clinical Progression.Biomedicines · 2020Article
- Salivary proteotypes of gingivitis tolerance and resilience.Journal of clinical periodontology · 2020Article
- Three-DimensionalTissue engineering. Part B, Reviews · 2020Review
- A review of co-culture models to study the oral microenvironment and disease.Journal of oral microbiology · 2020Review
- Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells.Scientific reports · 2019Article
- Recent progress in experimental and human disease-associated multi-species biofilms.Computational and structural biotechnology journal · 2019Review
- Oral Mucosal Epithelial Cells.Frontiers in immunology · 2019Review
- Saliva-Derived Commensal and Pathogenic Biofilms in a Human Gingiva Model.Journal of dental research · 2018Article
- Incorporation of staphylococci into titanium-grown biofilms: an in vitro "submucosal" biofilm model for peri-implantitis.Clinical oral implants research · 2016Article
- Proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Periodontitis is an infectious inflammatory disease that results in the destruction of the tooth-supporting (periodontal) tissues. The Gram-negative anaerobic species Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola, (also known as the "red complex" species) are highly associated with subgingival biofilms at periodontitis-affected sites. A major chemokine produced by the gingival epithelium in response to biofilm challenge, is interleukin (IL)-8. The aim of this in vitro study was to investigate the relative effect of the "red complex" species as constituents of subgingival biofilms, on the regulation of IL-8 by gingival epithelia. Multi-layered organotypic human gingival epithelial cultures were challenged with a 10-species in vitro subgingival biofilm model, or its 7-species variant, excluding the "red complex". IL-8 gene expression and secretion analyses were performed by qPCR and ELISA, respectively. After 3 h, both biofilms up-regulated IL-8 gene expression, but the presence of the "red complex" resulted in 3-fold greater response. IL-8 secretion was also up-regulated by both biofilms, with no differences between them. After 24 h, the 10-species biofilm reduced IL-8 secretion to 50% of the control, but this was not affected when the "red complex" was absent. In conclusion, as part of biofilms, "red complex" species differentially regulate IL-8 in gingival epithelia, potentially affecting the chemotactic responses of the tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.