Evidence map›Paper›PMID 24338737›Full record

ArticleEuropean journal of immunology2014

Self-priming determines high type I IFN production by plasmacytoid dendritic cells.

Sarah Kim, Vera Kaiser, Esther Beier, Matthias Bechheim, Margit Guenthner-Biller, Andrea Ablasser, Michael Berger, Stefan Endres, Gunther Hartmann, Veit Hornung

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 75 citations in OpenAlex.

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  11. The Influence of Sex Hormones and X Chromosome in Immune Responses.Current topics in microbiology and immunology · 2023
    Review
  12. Article
  13. Article
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  16. Ly6DNature communications · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Sarah KimInstitute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
Vera KaiserInstitute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
Esther BeierInstitute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
Matthias BechheimInstitute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
Margit Guenthner-BillerCenter of Integrated Protein Science (CIPS-M), Division of Clinical Pharmacology, Medizinische Klinik und Poliklinik IV, University of Munich, 80336 Munich, Germany.
Andrea AblasserInstitute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
Michael BergerCenter of Integrated Protein Science (CIPS-M), Division of Clinical Pharmacology, Medizinische Klinik und Poliklinik IV, University of Munich, 80336 Munich, Germany.
Stefan EndresCenter of Integrated Protein Science (CIPS-M), Division of Clinical Pharmacology, Medizinische Klinik und Poliklinik IV, University of Munich, 80336 Munich, Germany.
Gunther Hartmann *Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
Veit Hornung *Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53105 Bonn, Germany.
University of Bonn · DECenter for Integrated Protein Science Munich · DEUniversity Hospital Bonn · DE

Funding

European Research Council 243046
6 · The paper itself

Abstract

Plasmacytoid dendritic cells (pDCs) are responsible for the robust and immediate production of type I IFNs during viral infection. pDCs employ TLR7 and TLR9 to detect RNA and CpG motifs present in microbial genomes. CpG-A was the first synthetic stimulus available that induced large amounts of IFN-α (type I IFN) in pDCs. CpG-B, however, only weakly activates pDCs to produce IFN-α. Here, we demonstrate that differences in the kinetics of TLR9 activation in human pDCs are essential for the understanding of the functional difference between CpG-A and CpG-B. While CpG-B quickly induces IFN-α production in pDCs, CpG-A stimulation results in delayed yet maximal IFN-α induction. Constitutive production of low levels of type I IFN in pDCs, acting in a paracrine and autocrine fashion, turned out to be the key mechanism responsible for this phenomenon. At high cell density, pDC-derived, constitutive type I IFN production primes pDCs for maximal TLR responsiveness. This accounts for the high activity of higher structured TLR agonists that trigger type I IFN production in a delayed fashion. Altogether, these data demonstrate that high type I IFN production by pDCs cannot be simply ascribed to cell-autonomous mechanisms, yet critically depends on the local immune context.

Indexed as

Cells, CulturedCulture Media, ConditionedDendritic CellsHumansInterferon-alphaInterferon Type IKineticsLigandsOligodeoxyribonucleotidesToll-Like ReceptorsCulture Media, ConditionedInterferon-alphaInterferon Type ILigandsOligodeoxyribonucleotidesToll-Like ReceptorsCpG-ACpG-BpDCTLR9Type I IFN

Identifiers

PMID24338737
PMCPMC4523003
OpenAlexW2166990341

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.