ArticleAmerican journal of physiology. Lung cellular and molecular physiology2014
Fibroblast engraftment in the decellularized mouse lung occurs via a β1-integrin-dependent, FAK-dependent pathway that is mediated by ERK and opposed by AKT.
Article in American journal of physiology. Lung cellular and molecular physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- [Science advances · 2024Article
- Engineered Lung Tissues Prepared from Decellularized Lung Slices.Journal of visualized experiments : JoVE · 2022Article
- Halogen exposure injury in the developing lung.Annals of the New York Academy of Sciences · 2020Review
- Quantifying extracellular matrix turnover in human lung scaffold cultures.Scientific reports · 2018Article
- Exposure of neonatal mice to bromine impairs their alveolar development and lung function.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- New advances in the development of sarcoidosis models: a synopsis of a symposium sponsored by the Foundation for Sarcoidosis Research.Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG · 2018Article
- Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung.JCI insight · 2017Article
- Collagen I promotes hepatocellular carcinoma cell proliferation by regulating integrin β1/FAK signaling pathway in nonalcoholic fatty liver.Oncotarget · 2017Article
- Can We Understand the Pathobiology of Bronchopulmonary Dysplasia?The Journal of pediatrics · 2017Review
- Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Netrin-1 Regulates Fibrocyte Accumulation in the Decellularized Fibrotic Sclerodermatous Lung Microenvironment and in Bleomycin-Induced Pulmonary Fibrosis.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- Matrix-driven Myosin II Mediates the Pro-fibrotic Fibroblast Phenotype.The Journal of biological chemistry · 2016Article
- Revascularization of decellularized lung scaffolds: principles and progress.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- A critical review of the American Journal of Physiology-Lung Cellular and Molecular Physiology: 2012-2015.American journal of physiology. Lung cellular and molecular physiology · 2014Review
- Synergistic effect of interleukin-6 and hyaluronic acid on cell migration and ERK activation in human keratinocytes.Journal of Korean medical science · 2014Article
- Chitinase 3-like 1 suppresses injury and promotes fibroproliferative responses in Mammalian lung fibrosis.Science translational medicine · 2014Article
- Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells.Biomaterials · 2014Article
- Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Creation of bioartificial organs has been enhanced by the development of strategies involving decellularized mammalian lung. Because fibroblasts critically support lung function through a number of mechanisms, study of these cells in the context of the decellularized lung has the potential to improve the structure and function of tissue-engineered lungs. We characterized the engraftment and survival of a mouse fibroblast cell line in decellularized rat lung slices and found a time-dependent increase in cell numbers assessed by hematoxylin and eosin staining, cell proliferation assessed by Ki67 staining, and minimal cell death assessed by TUNEL staining. We developed a repopulation index to allow quantification of cell survival that accounts for variation in cell density throughout the seeded scaffold. We then applied this method to the study of mouse lung scaffolds and found that decellularization of presliced mouse lungs produced matrices with preserved alveolar architecture and proteinaceous components including fibronectin, collagens I and IV, laminin, and elastin. Treatment with a β1-integrin-neutralizing antibody significantly reduced the repopulation index after 24 h of culture. Treatment with focal adhesion kinase (FAK) inhibitor and extracellular signal-regulated kinase (ERK) inhibitor further reduced initial repopulation scores while treatment with AKT inhibitor increased initial scores. Rho-associated kinase inhibitor had no discernible effect. These data indicate that initial adhesion and survival of mouse fibroblasts in the decellularized mouse lung occur in a β1-integrin-dependent, FAK/ERK-dependent manner that is opposed by AKT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.