Evidence map›Paper›PMID 24337859›Full record

ArticleJournal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban2013

Morphological and molecular identification of two strains of dermatophytes.

Li Chen, Guo-You Shi, Mei-Mei Wang, Lin-Lin Zhao, Yuan-Yong Huang, Xiao-Lei Chen, Li-Jie Yuan, Ya-Nan Xiong, Dao-Wen Si, Li-Hua Zhu

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Article in Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Li ChenCollege of Clinical Medicine, Hebei United University, Tangshan, 063000, China.
Guo-You ShiJitang College, Hebei United University, Tangshan, 063000, China.
Mei-Mei WangDepartment of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
Lin-Lin ZhaoDepartment of Pharmacy, Pharmaceutical College, Hebei United University, Tangshan, 063000, China.
Yuan-Yong HuangDepartment of Biotechnology, College of Life Sciences, Hebei United University, Tangshan, 063000, China.
Xiao-Lei ChenCollege of Clinical Medicine, Hebei United University, Tangshan, 063000, China.
Li-Jie YuanDepartment of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
Ya-Nan XiongDepartment of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
Dao-Wen SiDepartment of Anatomy, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
Li-Hua ZhuDepartment of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China. zhulihua1972@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the microscope. And then the dermatophytes were cultured on Sabouraud's dextrose agar (SDA). The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction (PCR), and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN software were used to analyze the sequences. To further determine highly homologous sequences, a phylogenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosum and Microsporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate C115LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to microsporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR (JN650537, Cladosporium) and Ascomycete sp. LB68A1A2 (AY770409, Ascomycete sp).

Indexed as

ArthrodermataceaeHumansHyphaeRNA, FungalRNA, Ribosomal, 18SSkinRNA, FungalRNA, Ribosomal, 18S

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.