ArticleMicrobial ecology2014
High-throughput screening of multispecies biofilm formation and quantitative PCR-based assessment of individual species proportions, useful for exploring interspecific bacterial interactions.
Article in Microbial ecology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Enhanced Endophytic Colonization of Flavobacterium Promotes Plant Resistance to Fusarium Wilt.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Carbon substrate type shapes spatial self-organization in a multi-species biofilm community.The ISME journal · 2026Article
- Constraint-Based Metabolic Modeling Approach for Microbial Communities.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- Systematic screening of 42 vancomycin-resistant Enterococcus faecium strains for resistance, biofilm, and desiccation in simulated microgravity.NPJ microgravity · 2024Article
- Predatory protists impact plant performance by promoting plant growth-promoting rhizobacterial consortia.The ISME journal · 2024Article
- Interspecific interactions facilitate keystone species in a multispecies biofilm that promotes plant growth.The ISME journal · 2024Article
- Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-formingFrontiers in microbiology · 2024Article
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- A High-Throughput Microtiter Plate Screening Assay to Quantify and Differentiate Species in Dual-Species Biofilms.Microorganisms · 2023Article
- Phyllosphere-associated microbiota in built environment: Do they have the potential to antagonize human pathogens?Journal of advanced research · 2023Article
- THOR's Hammer: the Antibiotic Koreenceine Drives Gene Expression in a Model Microbial Community.mBio · 2022Article
- Enhanced Antibiotic Tolerance of anMicroorganisms · 2022Article
- Combined utilization of metabolic inhibitors to prevent synergistic multi-species biofilm formation.AMB Express · 2022Article
- Recent Updates on Microbial Biofilms in Periodontitis: An Analysis of In Vitro Biofilm Models.Advances in experimental medicine and biology · 2022Article
- Research progress in bioremediation of petroleum pollution.Environmental science and pollution research international · 2021Review
- We Are One: Multispecies Metabolism of a Biofilm Consortium and Their Treatment Strategies.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multispecies biofilms are predominant in almost all natural environments, where myriads of resident microorganisms interact with each other in both synergistic and antagonistic manners. The interspecies interactions among different bacteria are, despite the ubiquity of these communities, still poorly understood. Here, we report a rapid, reproducible and sensitive approach for quantitative screening of biofilm formation by bacteria when cultivated as mono- and multispecies biofilms, based on the Nunc-TSP lid system and crystal violet staining. The relative proportion of the individual species in a four-species biofilm was assessed using quantitative PCR based on SYBR Green I fluorescence with specific primers. The results indicated strong synergistic interactions in a four-species biofilm model community with a more than 3-fold increase in biofilm formation and demonstrated the strong dominance of two strains, Xanthomonas retroflexus and Paenibacillus amylolyticus. The developed approach can be used as a standard procedure for evaluating interspecies interactions in defined microbial communities. This will be of significant value in the quantitative study of the microbial composition of multispecies biofilms both in natural environments and infectious diseases to increase our understanding of the mechanisms that underlie cooperation, competition and fitness of individual species in mixed-species biofilms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.