ArticlePlant molecular biology2014
A novel chloroplast localized Rab GTPase protein CPRabA5e is involved in stress, development, thylakoid biogenesis and vesicle transport in Arabidopsis.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
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- Thylakostasis: key factors in thylakoid membrane organization with emphasis on biogenesis and remodeling proteins in vascular plants.Plant & cell physiology · 2025Review
- On the non-oxygenic origins of thylakoids.Communications biology · 2025Review
- Chloroplast Vesiculation and InducedPlant direct · 2025Article
- Three dimensional visualization of subcellular and suborganellar structures in near-native plant cells by synchrotron soft X-ray tomography.Frontiers in plant science · 2025Article
- Structure, biogenesis, and evolution of thylakoid membranes.The Plant cell · 2024Review
- Diversification of Plastid Structure and Function in Land Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Ultrastructural organization of the thylakoid system during the afternoon relocation of the giant chloroplast in Selaginella martensii Spring (Lycopodiophyta).Protoplasma · 2024Article
- Emerging role of small GTPases and their interactome in plants to combat abiotic and biotic stress.Protoplasma · 2023Review
- SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features.Nature communications · 2023Article
- Cyanobacterial membrane dynamics in the light of eukaryotic principles.Bioscience reports · 2023Article
- New perspectives on the molecular mechanisms of stress signalling by the nucleotide guanosine tetraphosphate (ppGpp), an emerging regulator of photosynthesis in plants and algae.The New phytologist · 2023Review
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2022Article
- NNature communications · 2022Article
- The vesicular trafficking system component MIN7 is required for minimizing Fusarium graminearum infection.Journal of experimental botany · 2021Article
- Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.Current genomics · 2021Review
- Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems.Frontiers in plant science · 2021Article
- A Sec14 domain protein is required for photoautotrophic growth and chloroplast vesicle formation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Exogenous DA-6 Improves the Low Night Temperature Tolerance of Tomato Through Regulating Cytokinin.Frontiers in plant science · 2020Article
- Chloroplasts- Beyond Energy Capture and Carbon Fixation: Tuning of Photosynthesis in Response to Chilling Stress.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloroplasts (stroma and thylakoids). Ypt31/32 in the yeast Saccharomyces cerevisiae are involved in regulating vesicle transport, and CPRabA5e a close homolog of Ypt31/32, restores the growth of the ypt31Δ ypt32(ts) mutant at 37 °C in yeast complementation. Knockout mutants of CPRabA5e displayed delayed seed germination and growth arrest during oxidative stress. Ultrastructural studies revealed that after preincubation at 4 °C mutant chloroplasts contained larger plastoglobules, lower grana, and more vesicles close to the envelopes compared to wild type, and vesicle formation being enhanced under oxidative stress. This indicated altered thylakoid development and organization of the mutants. A yeast-two-hybrid screen with CPRabA5e as bait revealed 13 interacting partner proteins, mainly located in thylakoids and plastoglobules. These proteins are known or predicted to be involved in development, stress responses, and photosynthesis related processes, consistent with the stress phenotypes observed. The results observed suggest a role of CPRabA5e in transport to and from thylakoids, similar to cytosolic Rab proteins involved in vesicle transport.
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