ArticleAmerican journal of physiology. Heart and circulatory physiology2014
NADH changes during hypoxia, ischemia, and increased work differ between isolated heart preparations.
Article in American journal of physiology. Heart and circulatory physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 63 citations in OpenAlex.
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- Combined emission ratiometry and motion tracking for optical mapping of contracting hearts: Validation with monophasic action potentials.The Journal of physiology · 2026Article
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- Multiparametric imaging of spatio-temporal cAMP signaling, transmembrane potential, and intracellular calcium in the intact heart.iScience · 2026Article
- Quadruple parametric optical mapping to measure cardiac metabolism-excitation-contraction coupling.Communications biology · 2025Article
- Effect of N-Acetylcysteine in Mitochondrial Function, Redox Signaling, and Sirtuin 3 Levels in the Heart During Cardiorenal Syndrome Type 4 Development.Antioxidants (Basel, Switzerland) · 2025Article
- Redefining the role of hypoxia-inducible factors (HIFs) in oxygen homeostasis.Communications biology · 2025Article
- Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.Journal of biomedical optics · 2024Article
- Review
- KairoSight-3.0: A validated optical mapping software to characterize cardiac electrophysiology, excitation-contraction coupling, and alternans.Journal of molecular and cellular cardiology plus · 2023Article
- Novel Imaging Technologies for Accurate Assessment of Cardiac Allograft Performance.Current transplantation reports · 2023Article
- Open thoracic surgical implantation of cardiac pacemakers in rats.Nature protocols · 2023Review
- Whole-heart multiparametric optical imaging reveals sex-dependent heterogeneity in cAMP signaling and repolarization kinetics.Science advances · 2023Article
- Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals.American journal of physiology. Heart and circulatory physiology · 2022Review
- Ectopic foci do not co-locate with ventricular epicardial stretch during early acute regional ischemia in isolated pig hearts.Physiological reports · 2022Article
- Ischemic damage to every segment of the oxidative phosphorylation cascade elevates ETC driving force and ROS production in cardiac mitochondria.American journal of physiology. Heart and circulatory physiology · 2022Article
- Inhibiting Succinate Release Worsens Cardiac Reperfusion Injury by Enhancing Mitochondrial Reactive Oxygen Species Generation.Journal of the American Heart Association · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Langendorff-perfused hearts and working hearts are established isolated heart preparation techniques that are advantageous for studying cardiac physiology and function, especially when fluorescence imaging is a key component. However, oxygen and energy requirements vary widely between isolated heart preparations. When energy supply and demand are not in harmony, such as when oxygen is not adequately available, the imbalance is reflected in NADH fluctuations. As such, NADH imaging can provide insight into the metabolic state of tissue. Hearts from New Zealand white rabbits were prepared as mechanically silenced Langendorff-perfused hearts, Langendorff-perfused hearts, or biventricular working hearts and subjected to sudden changes in workload, instantaneous global ischemia, and gradual hypoxia while heart rate, aortic pressure, and epicardial NADH fluorescence were monitored. Fast pacing resulted in a dip in NADH upon initiation and a spike in NADH when pacing was terminated in biventricular working hearts only, with the magnitude of the changes greatest at the fastest pacing rate. Working hearts were also most susceptible to changes in oxygen supply; NADH was at half-maximum value when perfusate oxygen was at 67.8 ± 13.7%. Langendorff-perfused and mechanically arrested hearts were the least affected by low oxygen supply, with half-maximum NADH occurring at 42.5 ± 5.0% and 23.7 ± 4.6% perfusate oxygen, respectively. Although the biventricular working heart preparation can provide a useful representation of mechanical in vivo heart function, it is not without limitations. Understanding the limitations of isolated heart preparations is crucial when studying cardiac function in the context of energy supply and demand.
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