ArticleBiology of reproduction2014
In vitro culture increases the frequency of stochastic epigenetic errors at imprinted genes in placental tissues from mouse concepti produced through assisted reproductive technologies.
Article in Biology of reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 136 citations in OpenAlex.
- Monocyte exhaustion associated with systemic lupus erythematosus.Autoimmunity · 2026Article
- Ultra-low oxygen tension duringbioRxiv : the preprint server for biology · 2026Article
- Impacts of Oxygen Tension on Developmental Competence of Preimplantation Embryos.Biomolecules · 2026Review
- Application of Chorionic Villus Sampling to Longitudinal Studies in Pregnant Non-Human Primate Models.Animals : an open access journal from MDPI · 2026Article
- The impact of melatonin-enriched media on epigenetic and perinatal changes induced by embryo culture in a mouse model.Journal of assisted reproduction and genetics · 2025Article
- In vitro fertilization induces reproductive changes in male mouse offspring and has multigenerational effects.JCI insight · 2025Article
- Review
- Placental expression and methylation of angiogenic factors in assisted reproductive technology pregnancies from India.Epigenomics · 2025Article
- Comparison of the developmental competence of in vitro-produced mouse embryos cultured under 5 versus 2% OJournal of assisted reproduction and genetics · 2024Article
- Cryopreservation, cryoprotectants, and potential risk of epigenetic alteration.Journal of assisted reproduction and genetics · 2024Review
- Iterative oxidation by TET1 is required for reprogramming of imprinting control regions and patterning of mouse sperm hypomethylated regions.Developmental cell · 2024Article
- Sperm DNA methylation defects in a new mouse model of the 5,10-methylenetetrahydrofolate reductase 677C>T variant and correction with moderate dose folic acid supplementation.Molecular human reproduction · 2024Article
- Altered DNA methylation underlies monocyte dysregulation and immune exhaustion memory in sepsis.Cell reports · 2024Article
- Trophectoderm biopsy of blastocysts following IVF and embryo culture increases epigenetic dysregulation in a mouse model.Human reproduction (Oxford, England) · 2024Article
- Contribution of the paternal histone epigenome to the preimplantation embryo.Frontiers in cell and developmental biology · 2024Review
- Higher incidence of embryonic defects in mouse offspring conceived with assisted reproduction from fathers with sperm epimutations.Human molecular genetics · 2023Article
- Long-Term Effects of ART on the Health of the Offspring.International journal of molecular sciences · 2023Review
- Unraveling the Consequences of Oxygen Imbalance on Early Embryo Development: Exploring Mitigation Strategies.Animals : an open access journal from MDPI · 2023Review
- Comparison of DNA methylation profiles of human embryos cultured in either uninterrupted or interrupted incubators.Journal of assisted reproduction and genetics · 2023Article
- Embryo cryopreservation leads to sex-specific DNA methylation perturbations in both human and mouse placentas.Human molecular genetics · 2022Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Assisted reproductive technologies (ART) have enabled millions of couples with compromised fertility to conceive children. Nevertheless, there is a growing concern regarding the safety of these procedures due to an increased incidence of imprinting disorders, premature birth, and low birth weight in ART-conceived offspring. An integral aspect of ART is the oxygen concentration used during in vitro development of mammalian embryos, which is typically either atmospheric (~20%) or reduced (5%). Both oxygen tension levels have been widely used, but 5% oxygen improves preimplantation development in several mammalian species, including that of humans. To determine whether a high oxygen tension increases the frequency of epigenetic abnormalities in mouse embryos subjected to ART, we measured DNA methylation and expression of several imprinted genes in both embryonic and placental tissues from concepti generated by in vitro fertilization (IVF) and exposed to 5% or 20% oxygen during culture. We found that placentae from IVF embryos exhibit an increased frequency of abnormal methylation and expression profiles of several imprinted genes, compared to embryonic tissues. Moreover, IVF-derived placentae exhibit a variety of epigenetic profiles at the assayed imprinted genes, suggesting that these epigenetic defects arise by a stochastic process. Although culturing embryos in both of the oxygen concentrations resulted in a significant increase of epigenetic defects in placental tissues compared to naturally conceived controls, we did not detect significant differences between embryos cultured in 5% and those cultured in 20% oxygen. Thus, further optimization of ART should be considered to minimize the occurrence of epigenetic errors in the placenta.
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