ArticleMolecular medicine reports2014
Nephrin and Podocin functions are highly conserved between the zebrafish pronephros and mammalian metanephros.
Article in Molecular medicine reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Loss of Arhgap24 alters glomerular ultrastructure in zebrafish model.Clinical and experimental nephrology · 2026Article
- Review
- Proximity-based proteomics (BioID) uncovers the Rho GTPase interactome in kidney podocytes.Frontiers in cell and developmental biology · 2025Article
- Melittin induces autophagy to alleviate chronic renal failure in 5/6-nephrectomized rats and angiotensin II-induced damage in podocytes.Nutrition research and practice · 2024Article
- Principles of Zebrafish Nephron Segment Development.Journal of developmental biology · 2023Review
- Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish.Journal of developmental biology · 2023Review
- A novel nanoluciferase transgenic reporter measures proteinuria in zebrafish.Kidney international · 2022Article
- A zebrafish model of congenital nephrotic syndrome of the Finnish type.Frontiers in cell and developmental biology · 2022Article
- Genetic compensation byCell cycle (Georgetown, Tex.) · 2019Article
- Ethanol Extract ofThe Malaysian journal of medical sciences : MJMS · 2019Article
- The human nephrin YThe Journal of biological chemistry · 2019Article
- Triptolide attenuates proteinuria and podocyte apoptosis via inhibition of NF-κB/GADD45B.Scientific reports · 2018Article
- Novel Microscopic Techniques for Podocyte Research.Frontiers in endocrinology · 2018Review
- Zebrafish as a Model for Drug Screening in Genetic Kidney Diseases.Frontiers in pediatrics · 2018Review
- GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway.Cell death & disease · 2016Article
- Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin.PloS one · 2016Article
- CRB2 mutations produce a phenotype resembling congenital nephrosis, Finnish type, with cerebral ventriculomegaly and raised alpha-fetoprotein.American journal of human genetics · 2015Article
- Using zebrafish to study podocyte genesis during kidney development and regeneration.Genesis (New York, N.Y. : 2000) · 2014Review
- Expression of Nephrin Homologue in the Freshwater Planarian, Dugesia japonica.Acta histochemica et cytochemica · 2014Article
- Anhuienoside C Attenuates Podocyte Injury in Diabetic Nephropathy Rats.Dose-response : a publication of International Hormesis SocietyArticle
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The slit diaphragm (SD) is a highly specialized intercellular junction between podocyte foot processes and is crucial in the formation of the filtration barrier in the renal glomeruli. Zebrafish Nephrin and Podocin are important in the formation of the podocyte SD and mutations in NEPHRIN and PODOCIN genes cause human nephrotic syndrome. In the present study, the zebrafish Podocin protein was observed to be predominantly localized in the pronephric glomerular podocytes, as previously reported for Nephrin. To understand the function of Podocin and Nephrin in zebrafish, splice‑blocking morpholino antisense oligonucleotides were used. Knockdown of Podocin or Nephrin by this method induced pronephric glomerular hypoplasia with pericardial edema. Human Nephrin and Podocin mRNA rescued this glomerular phenotype, however, the efficacy of the rescues was greatly reduced when mRNA‑encoding human disease‑causing NEPHRIN‑R1109X and PODOCIN‑R138Q were used. Furthermore, an association between zebrafish Nephrin and Podocin proteins was observed. Notably, Podocin‑R150Q, corresponding to human PODOCIN‑R138Q, markedly interacted with Nephrin compared with wild‑type Podocin, suggesting that this strong binding capacity of mutated Podocin impairs the transport of Nephrin and Podocin out of the endoplasmic reticulum. The results suggest that the functions of Nephrin and Podocin are highly conserved between the zebrafish pronephros and mammalian metanephros. Accordingly, the zebrafish pronephros may provide a useful tool for analyzing disease‑causing gene mutations in human kidney disorders.
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