ArticleIntegrative biology : quantitative biosciences from nano to macro2014
Single cell gene expression analysis in injury-induced collective cell migration.
Article in Integrative biology : quantitative biosciences from nano to macro, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Effects of a Hydrolyzed Lignin Derivative on Bleomycin-Induced Pulmonary Fibrosis in Mice.Bulletin of experimental biology and medicine · 2024Article
- Nrf2 Modulates the Hybrid Epithelial/Mesenchymal Phenotype and Notch Signaling During Collective Cancer Migration.Frontiers in molecular biosciences · 2022Article
- Curcumin upregulates transforming growth factor-β1, its receptors, and vascular endothelial growth factor expressions in an in vitro human gingival fibroblast wound healing model.BMC oral health · 2021Article
- Decoding leader cells in collective cancer invasion.Nature reviews. Cancer · 2021Review
- Curcumin Promotes Collagen Type I, Keratinocyte Growth Factor-1, and Epidermal Growth Factor Receptor Expressions in the In Vitro Wound Healing Model of Human Gingival Fibroblasts.European journal of dentistry · 2021Article
- NRF2-dependent Epigenetic Regulation can Promote the Hybrid Epithelial/Mesenchymal Phenotype.Frontiers in cell and developmental biology · 2021Article
- Partial EMT in Squamous Cell Carcinoma: A Snapshot.International journal of biological sciences · 2021Review
- Understanding the Principles of Pattern Formation Driven by Notch Signaling by Integrating Experiments and Theoretical Models.Frontiers in physiology · 2020Review
- A novel magnet-based scratch method for standardisation of wound-healing assays.Scientific reports · 2019Article
- NRF2 activates a partial epithelial-mesenchymal transition and is maximally present in a hybrid epithelial/mesenchymal phenotype.Integrative biology : quantitative biosciences from nano to macro · 2019Article
- A gapmer aptamer nanobiosensor for real-time monitoring of transcription and translation in single cells.Biomaterials · 2018Article
- Probing 3D Collective Cancer Invasion Using Double-Stranded Locked Nucleic Acid Biosensors.Analytical chemistry · 2016Article
- Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement.Scientific reports · 2016Article
- An Essential Role of NRF2 in Diabetic Wound Healing.Diabetes · 2016Article
- Notch1-Dll4 signalling and mechanical force regulate leader cell formation during collective cell migration.Nature communications · 2015Article
- Article
- Probing mechanoregulation of neuronal differentiation by plasma lithography patterned elastomeric substrates.Scientific reports · 2014Article
- Mapping photothermally induced gene expression in living cells and tissues by nanorod-locked nucleic acid complexes.ACS nano · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Collective cell behavior in response to mechanical injury is central to various regenerative and pathological processes. Using a double-stranded locked nucleic acid probe for monitoring real-time intracellular gene expression, we examined the spatiotemporal response of epithelial cells during injury-induced collective migration and compared to the blocker assay with minimal injury as control. We showed that cells ∼150 μm from the wound edge exhibit a gradient in response to mechanical injury, expressing different genes depending on the wounding process. While release of contact inhibition is sufficient to trigger the migratory behavior, cell injury additionally induces reactive oxygen species, Nrf2 protein, and stress response genes, including heat shock protein 70 and heme oxygenase-1, in a spatiotemporal manner. Furthermore, we show that Nrf2 has an inhibitory role in injury-induced epithelial-mesenchymal transition, suggesting a potential autoregulatory mechanism in injury-induced response. Taken together, our single-cell gene expression analyses reveal modular cell responses to mechanical injury, manipulation of which may afford novel strategies for tissue repair and prevention of tumor invasion in the future.
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