ArticleGenome research2014
Principles of nucleation of H3K27 methylation during embryonic development.
Article in Genome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 79 citations in OpenAlex.
- H3K27ac Is Essential for Human Naive Pluripotency Modulated by m6A-Driven EP300 Expression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Foxi2 and Sox3 are master transcription regulators that control ectoderm germ layer specification in Xenopus.PLoS biology · 2025Article
- Foxh1 is a locus-specific PRC2 recruiter governing germ layer silencing.bioRxiv : the preprint server for biology · 2025Article
- The rebirth of repressive chromatin during early vertebrate development.Annals of the New York Academy of Sciences · 2025Review
- Sperm derived H2AK119ub1 is required for embryonic development in Xenopus laevis.Nature communications · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- DNA methylation clocks for clawed frogs reveal evolutionary conservation of epigenetic aging.GeroScience · 2024Article
- Article
- Epigenomic dynamics of early Xenopus Embryos.Development, growth & differentiation · 2022Review
- Review
- EED is required for mouse primordial germ cell differentiation in the embryonic gonad.Developmental cell · 2022Article
- Gnocis: An integrated system for interactive and reproducible analysis and modelling of cis-regulatory elements in Python 3.PloS one · 2022Article
- Loss of PRC2 subunits primes lineage choice during exit of pluripotency.Nature communications · 2021Article
- The chromatin, topological and regulatory properties of pluripotency-associated poised enhancers are conserved in vivo.Nature communications · 2021Article
- MOCCA: a flexible suite for modelling DNA sequence motif occurrence combinatorics.BMC bioinformatics · 2021Article
- Control of zygotic genome activation in Xenopus.Current topics in developmental biology · 2021Review
- DNA sequence models of genome-wide Drosophila melanogaster Polycomb binding sites improve generalization to independent Polycomb Response Elements.Nucleic acids research · 2019Article
- Advancing genetic and genomic technologies deepen the pool for discovery in Xenopus tropicalis.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation.Cell reports · 2019Article
- Towards understanding of PRC2 binding to RNA.RNA biology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
During embryonic development, maintenance of cell identity and lineage commitment requires the Polycomb-group PRC2 complex, which catalyzes histone H3 lysine 27 trimethylation (H3K27me3). However, the developmental origins of this regulation are unknown. Here we show that H3K27me3 enrichment increases from blastula stages onward in embryos of the Western clawed frog (Xenopus tropicalis) within constrained domains strictly defined by sequence. Strikingly, although PRC2 also binds widely to active enhancers, H3K27me3 is only deposited at a small subset of these sites. Using a Support Vector Machine algorithm, these sequences can be predicted accurately on the basis of DNA sequence alone, with a sequence signature conserved between humans, frogs, and fish. These regions correspond to the subset of blastula-stage DNA methylation-free domains that are depleted for activating promoter motifs, and enriched for motifs of developmental factors. These results imply a genetic-default model in which a preexisting absence of DNA methylation is the major determinant of H3K27 methylation when not opposed by transcriptional activation. The sequence and motif signatures reveal the hierarchical and genetically inheritable features of epigenetic cross-talk that impose constraints on Polycomb regulation and guide H3K27 methylation during the exit of pluripotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.