ArticleAmerican journal of physiology. Cell physiology2014
Conditions that promote primary human skeletal myoblast culture and muscle differentiation in vitro.
Article in American journal of physiology. Cell physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Pooled it
- Evaluation of material effects on three-dimensional cultured skeletal muscle cells for biohybrid robots.Frontiers in robotics and AI · 2026Article
- Decellularized extracellular matrix scaffolds fromMaterials today. Bio · 2025Article
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- Selection of optimal human myoblasts based on patient related factors influencing proliferation and differentiation capacity.Scientific reports · 2025Article
- Intrinsic signalling factors associated with cancer cell-cell fusion.Cell communication and signaling : CCS · 2023Review
- Serum concentration impacts myosin heavy chain expression but not cellular respiration in human LHCN-M2 myoblasts undergoing differentiation.Experimental physiology · 2023Article
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- Impact of Cell Seeding Density and Cell Confluence on Human Tissue Engineered Skeletal Muscle.Tissue engineering. Part A · 2022Article
- In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging.eLife · 2022Article
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- The miR-133a, TPM4 and TAp63γ Role in Myocyte Differentiation Microfilament Remodelling and Colon Cancer Progression.International journal of molecular sciences · 2021Article
- Engineering skeletal muscle tissues with advanced maturity improves synapse formation with human induced pluripotent stem cell-derived motor neurons.APL bioengineering · 2021Article
- Article
- Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes.Scientific reports · 2020Article
- Neuromuscular disease modeling on a chip.Disease models & mechanisms · 2020Review
- Phosphorylated GSK‑3β protects stress‑induced apoptosis of myoblasts via the PI3K/Akt signaling pathway.Molecular medicine reports · 2020Article
- A multiplexed in vitro assay system for evaluating human skeletal muscle functionality in response to drug treatment.Biotechnology and bioengineering · 2020Article
- Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA.Journal of visualized experiments : JoVE · 2020Article
- Effects of conditioned media from murine lung cancer cells and human tumor cells on cultured myotubes.American journal of physiology. Endocrinology and metabolism · 2020Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Conditions under which skeletal myoblasts are cultured in vitro are critical to growth and differentiation of these cells into mature skeletal myofibers. We examined several culture conditions that promoted human skeletal myoblast (HSkM) culture and examined the effect of microRNAs and mechanical stimulation on differentiation. Culture conditions for HSkM are different from those that enable rapid C2C12 myoblast differentiation. Culture on a growth factor-reduced Matrigel (GFR-MG)-coated surface in 2% equine serum-supplemented differentiation medium to promote HSkM differentiation under static conditions was compared with culture conditions used for C2C12 cell differentiation. Such conditions led to a >20-fold increase in myogenic miR-1, miR-133a, and miR-206 expression, a >2-fold increase in myogenic transcription factor Mef-2C expression, and an increase in sarcomeric α-actinin protein. Imposing ±10% cyclic stretch at 0.5 Hz for 1 h followed by 5 h of rest over 2 wk produced a >20% increase in miR-1, miR-133a, and miR-206 expression in 8% equine serum and a >35% decrease in 2% equine serum relative to static conditions. HSkM differentiation was accelerated in vitro by inhibition of proliferation-promoting miR-133a: immunofluorescence for sarcomeric α-actinin exhibited accelerated development of striations compared with the corresponding negative control, and Western blotting showed 30% more α-actinin at day 6 postdifferentiation. This study showed that 100 μg/ml GFR-MG coating and 2% equine serum-supplemented differentiation medium enhanced HSkM differentiation and myogenic miR expression and that addition of antisense miR-133a alone can accelerate primary human skeletal muscle differentiation in vitro.
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