ArticleJournal of visualized experiments : JoVE2013
Microfluidic picoliter bioreactor for microbial single-cell analysis: fabrication, system setup, and operation.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Guideline
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- Development of a Mass-Producible Microfluidic Device for Single and Bulk Mycobacteria Investigations.Biosensors · 2025Article
- Time-resolved cell-to-cell heterogeneity ofFrontiers in bioengineering and biotechnology · 2024Article
- Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis.Frontiers in microbiology · 2023Article
- Assessing the growth kinetics and stoichiometry ofEngineering in life sciences · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis.Microbial cell factories · 2021Article
- Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit.Molecular systems biology · 2021Article
- Inducible Expression Systems Based on Xenogeneic Silencing and Counter-Silencing and Design of a Metabolic Toggle Switch.ACS synthetic biology · 2020Article
- Genetically Encoded Photosensitizers as Light-Triggered Antimicrobial Agents.International journal of molecular sciences · 2019Article
- Generation of a Prophage-Free Variant of the Fast-Growing Bacterium Vibrio natriegens.Applied and environmental microbiology · 2019Article
- Microbial single-cell growth response at defined carbon limiting conditions.RSC advances · 2019Article
- Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips.Microorganisms · 2019Article
- Importance of Pyruvate Sensing and Transport for the Resuscitation of Viable but NonculturableJournal of bacteriology · 2019Article
- A microfluidic photobioreactor for simultaneous observation and cultivation of single microalgal cells or cell aggregates.PloS one · 2019Article
- Increased Mortality in Mice following Immunoprophylaxis Therapy with High Dosage of Nicotinamide inInfection and immunity · 2019Article
- Cytometry meets next-generation sequencing - RNA-Seq of sorted subpopulations reveals regional replication and iron-triggered prophage induction in Corynebacterium glutamicum.Scientific reports · 2018Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this protocol the fabrication, experimental setup and basic operation of the recently introduced microfluidic picoliter bioreactor (PLBR) is described in detail. The PLBR can be utilized for the analysis of single bacteria and microcolonies to investigate biotechnological and microbiological related questions concerning, e.g. cell growth, morphology, stress response, and metabolite or protein production on single-cell level. The device features continuous media flow enabling constant environmental conditions for perturbation studies, but in addition allows fast medium changes as well as oscillating conditions to mimic any desired environmental situation. To fabricate the single use devices, a silicon wafer containing sub micrometer sized SU-8 structures served as the replication mold for rapid polydimethylsiloxane casting. Chips were cut, assembled, connected, and set up onto a high resolution and fully automated microscope suited for time-lapse imaging, a powerful tool for spatio-temporal cell analysis. Here, the biotechnological platform organism Corynebacterium glutamicum was seeded into the PLBR and cell growth and intracellular fluorescence were followed over several hours unraveling time dependent population heterogeneity on single-cell level, not possible with conventional analysis methods such as flow cytometry. Besides insights into device fabrication, furthermore, the preparation of the preculture, loading, trapping of bacteria, and the PLBR cultivation of single cells and colonies is demonstrated. These devices will add a new dimension in microbiological research to analyze time dependent phenomena of single bacteria under tight environmental control. Due to the simple and relatively short fabrication process the technology can be easily adapted at any microfluidics lab and simply tailored towards specific needs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.