Evidence map›Paper›PMID 24336077›Full record

ArticleCell death & disease2013

Mycobacterial HBHA induces endoplasmic reticulum stress-mediated apoptosis through the generation of reactive oxygen species and cytosolic Ca2+ in murine macrophage RAW 264.7 cells.

J-A Choi, Y-J Lim, S-N Cho, J-H Lee, J A Jeong, E J Kim, J B Park, S H Kim, H S Park, H-J Kim and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 76 citations in OpenAlex.

  1. The microbiota-proteostasis axis: implications in neurodegenerative diseases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2026
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  12. Emerging microbes & infections · 2021
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  14. New Insights into the Methylation ofPathogens (Basel, Switzerland) · 2021
    Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
  20. TLR-2 mediated cytosolic-CaScientific reports · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

J-A Choi1] Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea.
Y-J Lim1] Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea.
S-N Cho1] Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea.
J-H Lee1] Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea.
J A JeongDepartment of Physiology, College of Medicine, Chungnam National University, Daejeon, South Korea.
E J KimDepartment of Physiology, College of Medicine, Konyang University, Daejeon, South Korea.
J B Park1] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Department of Physiology, College of Medicine, Chungnam National University, Daejeon, South Korea [3] Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, South Korea.
S H KimDepartment of Physiology, College of Medicine, Konyang University, Daejeon, South Korea.
H S ParkDepartment of Physiology, College of Medicine, Konyang University, Daejeon, South Korea.
H-J Kim1] Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea [3] Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, South Korea.
C-H Song1] Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, South Korea [2] Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea [3] Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, South Korea.
Chungnam National University · KRKonyang University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycobacterial heparin-binding haemagglutinin antigen (HBHA) is a virulence factor that induces apoptosis of macrophages. Endoplasmic reticulum (ER) stress-mediated apoptosis is an important regulatory response that can be utilised to study the pathogenesis of tuberculosis. In the present study, HBHA stimulation induced ER stress sensor molecules in a caspase-dependent manner. Pre-treatment of RAW 264.7 cells with an IκB kinase 2 inhibitor reduced not only C/EBP homology protein expression but also IL-6 and monocyte chemotactic protein-1 (MCP-1) production. BAPTA-AM reduced both ER stress responses and caspase activation and strongly suppressed HBHA-induced IL-6 and MCP-1 production in RAW 264.7 cells. Enhanced reactive oxygen species (ROS) production and elevated cytosolic [Ca(2+)]i levels were essential for HBHA-induced ER stress responses. Collectively, our data suggest that HBHA induces cytosolic [Ca(2+)]i, which influences the generation of ROS associated with the production of proinflammatory cytokines. These concerted and complex cellular responses induce ER stress-associated apoptosis during HBHA stimulation in macrophages. These results indicate that the ER stress pathway has an important role in the HBHA-induced apoptosis during mycobacterial infection.

Indexed as

AnimalsApoptosisCalciumCell LineCytosolEndoplasmic Reticulum StressLectinsMacrophagesMiceReactive Oxygen SpeciesCalciumheparin-binding hemagglutininLectinsReactive Oxygen Species

Identifiers

PMID24336077
PMCPMC3877560
OpenAlexW2095255992

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.