ArticleCancers2013
Circulating Tumor Cell Detection and Capture by Photoacoustic Flow Cytometry in Vivo and ex Vivo.
Article in Cancers, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06488365 (In Vivo Liquid Biopsy for Early Detection of Metastatic Melanoma), which is not on this map. Cited by 61 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
In Vivo Liquid Biopsy for Early Detection of Metastatic Melanoma
Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 123 citations in OpenAlex.
- Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review.Journal of biomedical optics · 2024Pooled it
- Utilizing Magnetic Microraft Technology for Single-Cell Isolation of CTCs from Whole Blood for scRNA-Seq Transcriptomic Characterization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Efficient implementations of a Born Series for computing photoacoustic field from a collection of erythrocytes.Photoacoustics · 2025Article
- Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon.Nature communications · 2024Article
- Hypoxia induces ROS-resistant memory upon reoxygenation in vivo promoting metastasis in part via MUC1-C.Nature communications · 2024Article
- Heterogeneity and molecular landscape of melanoma: implications for targeted therapy.Molecular biomedicine · 2024Review
- Machine Learning Enabled Photoacoustic Spectroscopy for Noninvasive Assessment of Breast Tumor ProgressionACS sensors · 2024Article
- Dissemination of Circulating Tumor Cells in Breast and Prostate Cancer: Implications for Early Detection.Endocrinology · 2024Review
- Photoacoustic microscopy for real-time monitoring of near-infrared optical absorbers inside biological tissue.Journal of biomedical optics · 2024Article
- High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.Trends in analytical chemistry : TRAC · 2023Article
- Biogenic Imaging Contrast Agents.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- The sound of blood: photoacoustic imaging in blood analysis.Medicine in novel technology and devices · 2023Article
- Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria.Micromachines · 2023Article
- Article
- Multi-color flow cytometer with PIC-based structured illumination.Biomedical optics express · 2022Article
- Isolation of circulating tumor cells to diagnose melanoma and evaluate the efficacy of surgical resection using melanoma-specific microsystem.Advanced nanobiomed research · 2022Article
- Towards rainbow portable Cytophone with laser diodes for global disease diagnostics.Scientific reports · 2022Article
- Photoacoustic Imaging in Biomedicine and Life Sciences.Life (Basel, Switzerland) · 2022Review
- Photoacoustic discrimination of antibiotic-resistant and sensitive Staphylococcus aureus isolates.Lasers in surgery and medicine · 2022Article
- Emerging photoacoustic and thermoacoustic imaging technologies for detecting primary and metastatic cancer and guiding therapy.Clinical & experimental metastasis · 2022Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Despite progress in detecting circulating tumor cells (CTCs), existing assays still have low sensitivity (1-10 CTC/mL) due to the small volume of blood samples (5-10 mL). Consequently, they can miss up to 103-104 CTCs, resulting in the development of barely treatable metastasis. Here we analyze a new concept of in vivo CTC detection with enhanced sensitivity (up to 102-103 times) by the examination of the entire blood volume in vivo (5 L in adults). We focus on in vivo photoacoustic (PA) flow cytometry (PAFC) of CTCs using label-free or targeted detection, photoswitchable nanoparticles with ultrasharp PA resonances, magnetic trapping with fiber-magnetic-PA probes, optical clearance, real-time spectral identification, nonlinear signal amplification, and the integration with PAFC in vitro. We demonstrate PAFC's capability to detect rare leukemia, squamous carcinoma, melanoma, and bulk and stem breast CTCs and its clusters in preclinical animal models in blood, lymph, bone, and cerebrospinal fluid, as well as the release of CTCs from primary tumors triggered by palpation, biopsy or surgery, increasing the risk of metastasis. CTC lifetime as a balance between intravasation and extravasation rates was in the range of 0.5-4 h depending on a CTC metastatic potential. We introduced theranostics of CTCs as an integration of nanobubble-enhanced PA diagnosis, photothermal therapy, and feedback through CTC counting. In vivo data were verified with in vitro PAFC demonstrating a higher sensitivity (1 CTC/40 mL) and throughput (up to 10 mL/min) than conventional assays. Further developments include detection of circulating cancer-associated microparticles, and super-rsesolution PAFC beyond the diffraction and spectral limits.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.