ArticleProceedings of the National Academy of Sciences of the United States of America2014
Reaction-based fluorescent sensor for investigating mobile Zn2+ in mitochondria of healthy versus cancerous prostate cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it, 158 citations in OpenAlex.
- Comparative study of serum zinc concentrations in benign and malignant prostate disease: A Systematic Review and Meta-Analysis.Scientific reports · 2016Pooled it
- Present status of prostate cancer diagnosis, limitations, challenges, and future endeavors.Annals of medicine · 2026Review
- Reversible fluorescent solid porous films for detection of zinc ions in biological media.Journal of biological engineering · 2025Article
- Toward decoding spatiotemporal signaling activities of reactive immunometabolites with precision immuno-chemical biology tools.Communications chemistry · 2024Review
- A Naphthoquinoline-Dione-Based CuMolecules (Basel, Switzerland) · 2024Article
- Fluorescein-based sensors to purify human α-cells for functional and transcriptomic analyses.eLife · 2023Article
- A Semisynthetic Bioluminescence Sensor for Ratiometric Imaging of Metal Ions In Vivo Using DNAzymes Conjugated to An Engineered Nano-Luciferase.Angewandte Chemie (International ed. in English) · 2023Article
- Thermogenic adipocyte-derived zinc promotes sympathetic innervation in male mice.Nature metabolism · 2023Article
- Advances in reaction-based synthetic fluorescent probes for studying the role of zinc and copper ions in living systems.Journal of clinical biochemistry and nutrition · 2023Article
- Mobile zinc as a modulator of sensory perception.FEBS letters · 2023Review
- Zinc in Prostate Health and Disease: A Mini Review.Biomedicines · 2022Review
- Neuronal signalling of zinc: from detection and modulation to function.Open biology · 2022Review
- Rational Design of Silicon-Based Zinc Ionophores.Angewandte Chemie (International ed. in English) · 2022Article
- Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal IonsJournal of the American Chemical Society · 2022Article
- Brain-derived neurotrophic factor in Alzheimer's disease and its pharmaceutical potential.Translational neurodegeneration · 2022Review
- A Chromo-Fluorogenic Naphthoquinolinedione-Based Probe for Dual Detection of CuMolecules (Basel, Switzerland) · 2022Article
- Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells.Life science alliance · 2021Article
- Simultaneous ZnNature communications · 2021Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of β cells.Science advances · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Chelatable, mobile forms of divalent zinc, Zn(II), play essential signaling roles in mammalian biology. A complex network of zinc import and transport proteins has evolved to control zinc concentration and distribution on a subcellular level. Understanding the action of mobile zinc requires tools that can detect changes in Zn(II) concentrations at discrete cellular locales. We present here a zinc-responsive, reaction-based, targetable probe based on the diacetyled form of Zinpyr-1. The compound, (6-amidoethyl)triphenylphosphonium Zinpyr-1 diacetate (DA-ZP1-TPP), is essentially nonfluorescent in the metal-free state; however, exposure to Zn(II) triggers metal-mediated hydrolysis of the acetyl groups to afford a large, rapid, and zinc-induced fluorescence response. DA-ZP1-TPP is insensitive to intracellular esterases over a 2-h period and is impervious to proton-induced turn-on. A TPP unit is appended for targeting mitochondria, as demonstrated by live cell fluorescence imaging studies. The practical utility of DA-ZP1-TPP is demonstrated by experiments revealing that, in contrast to healthy epithelial prostate cells, tumorigenic cells are unable to accumulate mobile zinc within their mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.