ArticleMolecular & cellular proteomics : MCP2014
Proteome and phosphoproteome characterization reveals new response and defense mechanisms of Brachypodium distachyon leaves under salt stress.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 127 citations in OpenAlex.
- Fungal allies in saline soils: mineral mobilization, plant nutrient acquisition and salinity stress tolerance.Frontiers in microbiology · 2026Review
- Identification of thePlants (Basel, Switzerland) · 2025Article
- Twenty years of mining salt tolerance genes in soybean.Molecular breeding : new strategies in plant improvement · 2023Article
- Article
- 2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress.BMC genomics · 2022Article
- A Phosphoproteomics Study of the SoybeanFrontiers in plant science · 2022Article
- Endoplasmic Reticulum Subproteome Analysis Reveals Underlying Defense Mechanisms of Wheat Seedling Leaves under Salt Stress.International journal of molecular sciences · 2021Article
- Cellular Phosphorylation Signaling and Gene Expression in Drought Stress Responses: ABA-Dependent and ABA-Independent Regulatory Systems.Plants (Basel, Switzerland) · 2021Review
- Galactolipid and Phospholipid Profile and Proteome Alterations in Soybean Leaves at the Onset of Salt Stress.Frontiers in plant science · 2021Article
- Phosphoproteomic Analysis of Soybean Roots Under Salinity by Using the iTRAQ Labeling Approach.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Identification of stress defensive proteins in common wheat-Breeding science · 2020Article
- Screening of Salt Stress Responsive Genes inPlants (Basel, Switzerland) · 2020Article
- Proteomic responses to progressive dehydration stress in leaves of chickpea seedlings.BMC genomics · 2020Article
- NaGenomics, proteomics & bioinformatics · 2020Article
- Enhanced Salt Tolerance of Rhizobia-inoculated Soybean Correlates with Decreased Phosphorylation of the Transcription Factor GmMYB183 and Altered Flavonoid Biosynthesis.Molecular & cellular proteomics : MCP · 2019Article
- Comparative proteomics and gene expression analyses revealed responsive proteins and mechanisms for salt tolerance in chickpea genotypes.BMC plant biology · 2019Article
- Evolution of protein kinase substrate recognition at the active site.PLoS biology · 2019Article
- Conserved phosphorylation hotspots in eukaryotic protein domain families.Nature communications · 2019Article
- Comparative Transcriptomic Analysis of Biological Process and Key Pathway in Three Cotton (International journal of molecular sciences · 2019Article
- Identification of Early Salinity Stress-Responsive Proteins inInternational journal of molecular sciences · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salinity is a major abiotic stress affecting plant growth and development. Understanding the molecular mechanisms of salt response and defense in plants will help in efforts to improve the salt tolerance of crops. Brachypodium distachyon is a new model plant for wheat, barley, and several potential biofuel grasses. In the current study, proteome and phosphoproteome changes induced by salt stress were the focus. The Bd21 leaves were initially treated with salt in concentrations ranging from 80 to 320 mm and then underwent a recovery process prior to proteome analysis. A total of 80 differentially expressed protein spots corresponding to 60 unique proteins were identified. The sample treated with a median salt level of 240 mm and the control were selected for phosphopeptide purification using TiO2 microcolumns and LC-MS/MS for phosphoproteome analysis to identify the phosphorylation sites and phosphoproteins. A total of 1509 phosphoproteins and 2839 phosphorylation sites were identified. Among them, 468 phosphoproteins containing 496 phosphorylation sites demonstrated significant changes at the phosphorylation level. Nine phosphorylation motifs were extracted from the 496 phosphorylation sites. Of the 60 unique differentially expressed proteins, 14 were also identified as phosphoproteins. Many proteins and phosphoproteins, as well as potential signal pathways associated with salt response and defense, were found, including three 14-3-3s (GF14A, GF14B, and 14-3-3A) for signal transduction and several ABA signal-associated proteins such as ABF2, TRAB1, and SAPK8. Finally, a schematic salt response and defense mechanism in B. distachyon was proposed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.