ArticleJournal of virology2014
Characterization of human astrovirus cell entry.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
- Structure of the human astrovirus capsid spike in complex with the neonatal Fc receptor.Nature communications · 2025Article
- The novel human astrovirus VA1 requires the proteasome during cell entry.The Journal of general virology · 2025Article
- Functional characterization of DPP4 and FcRn as receptor and coreceptor for classical human astroviruses in Caco-2 cells.PLoS pathogens · 2025Article
- Unlocking the secrets of Feline calicivirus: advances in structural and nonstructural proteins and its role as a key model for other Caliciviruses.Virology journal · 2025Review
- Neonatal Fc receptor is a functional receptor for classical human astrovirus.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- VP2 mediates the release of the feline calicivirus RNA genome by puncturing the endosome membrane of infected cells.Journal of virology · 2024Article
- miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens.International journal of molecular sciences · 2023Article
- Human astrovirus capsid protein releases a membrane lytic peptide upon trypsin maturation.Journal of virology · 2023Article
- Structure of the divergent human astrovirus MLB capsid spike.Structure (London, England : 1993) · 2022Article
- Intra- and Cross-Species Transmission of Astroviruses.Viruses · 2021Review
- Review
- Structural Insights into the Human Astrovirus Capsid.Viruses · 2021Review
- Animal and human RNA viruses: genetic variability and ability to overcome vaccines.Archives of microbiology · 2021Review
- Protein Disulfide Isomerase A4 Is Involved in Genome Uncoating during Human Astrovirus Cell Entry.Viruses · 2020Article
- Article
- Identification of a Type-Specific Epitope in the ORF2 Protein of Duck Astrovirus Type 1.Animals : an open access journal from MDPI · 2019Article
- Astrovirus replication in human intestinal enteroids reveals multi-cellular tropism and an intricate host innate immune landscape.PLoS pathogens · 2019Article
- Early Transcriptional Response to DNA Virus Infection inViruses · 2019Article
- Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.Frontiers in microbiology · 2019Review
- Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis.Archives of virology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human astroviruses (HAstV) are a frequent cause of gastroenteritis in young children and immunocompromised patients. To understand the early steps of HAstV infection in the highly permissive Caco-2 cell line, the binding and entry processes of the virus were characterized. The half-time of virus binding to the cell surface was about 10 min, while virus decapsidation took around 130 min. Drugs affecting clathrin-mediated endocytosis, endosome acidification, and actin filament polymerization, as well as those that reduce the presence of cholesterol in the cell membrane, decreased the infectivity of the virus. The infection was also reduced by silencing the expression of the clathrin heavy chain (CHC) by RNA interference or by overexpression of dominant-negative mutants of dynamin 2 and Eps15. Furthermore, the entry of HAstV apparently depends on the maturation of endosomes, since the infection was reduced by silencing the expression of Rab7, a small GTPase involved in the early- to late-endosome maturation. Altogether, our results suggest that HAstV enters Caco-2 cells using a clathrin-dependent pathway and reaches late endosomes to enter cells. Here, we have characterized the mechanism used by human astroviruses, important agents of gastroenteritis in children, to gain entry into their host cells. Using a combination of biochemical and genetic tools, we found that these viruses enter Caco-2 cells using a clathrin-dependent endocytic pathway, where they most likely need to travel to late endosomes to reach the cytoplasm and begin their replication cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.