ArticleApplied and environmental microbiology2014
Functional analysis of environmental DNA-derived microviridins provides new insights into the diversity of the tricyclic peptide family.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Secondary metabolism ofApplied and environmental microbiology · 2026Review
- Discovery, Function, and Engineering of Graspetides.Trends in chemistry · 2023Article
- Recent Advances in Discovery, Bioengineering, and Bioactivity-Evaluation of Ribosomally Synthesized and Post-translationally Modified Peptides.ACS bio & med chem Au · 2023Review
- One-Pot Chemoenzymatic Synthesis of Microviridin Analogs Containing Functional Tags.Chembiochem : a European journal of chemical biology · 2022Article
- Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides.Chemical reviews · 2022Review
- Heterologous expression of a cryptic gene cluster from Marinomonas fungiae affords a novel tricyclic peptide marinomonasin.Applied microbiology and biotechnology · 2021Article
- First Report onToxins · 2021Article
- Comparative Genomics Analysis of Keratin-DegradingMicroorganisms · 2021Article
- Current Knowledge on Microviridin from Cyanobacteria.Marine drugs · 2021Review
- Microviridin 1777: A Toxic Chymotrypsin Inhibitor Discovered by a Metabologenomic Approach.Journal of natural products · 2020Article
- Illumina sequencing and assessment of new cost-efficient protocol for metagenomic-DNA extraction from environmental water samples.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2018Article
- Natural products chemistry: The emerging trends and prospective goals.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2018Article
- Comparison of Strategies to Overcome Drug Resistance: Learning from Various Kingdoms.Molecules (Basel, Switzerland) · 2018Review
- Metagenomics and Bioinformatics in Microbial Ecology: Current Status and Beyond.Microbes and environments · 2016Review
- The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.Microbial cell factories · 2016Review
- Emerging concepts promising new horizons for marine biodiscovery and synthetic biology.Marine drugs · 2015Review
- Mining the metabiome: identifying novel natural products from microbial communities.Chemistry & biology · 2014Review
- Metagenomic small molecule discovery methods.Current opinion in microbiology · 2014Article
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microviridins represent a unique family of ribosomally synthesized cage-like depsipeptides from cyanobacteria with potent protease-inhibitory activities. The natural diversity of these peptides is largely unexplored. Here, we describe two methodologies that were developed to functionally characterize cryptic microviridin gene clusters from metagenomic DNA. Environmental samples were collected and enriched from cyanobacterial freshwater blooms of different geographical origins containing predominantly Microcystis sp. Microviridins were produced either directly from fosmid clones or after insertion of environmental DNA-derived gene cassettes into a minimal expression platform in Escherichia coli. Three novel microviridin variants were isolated and tested against different serine-type proteases. The comparison of the bioactivity profiles of the new congeners allows deduction of further structure-function relationships for microviridins. Moreover, this study provides new insights into microviridin processing and gene cluster organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.