ArticleApplied and environmental microbiology2014
Deregulation of feedback inhibition of phosphoenolpyruvate carboxylase for improved lysine production in Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 101 citations in OpenAlex.
- Recent advances in the Design-Build-Test-Learn (DBTL) cycle for systems metabolic engineering of Corynebacterium glutamicum.Journal of microbiology (Seoul, Korea) · 2025Review
- A novel engineered strain of Methylorubrum extorquens for methylotrophic production of glycolic acid.Microbial cell factories · 2024Article
- Biotin concentration affects anaplerotic reactions functioning in glutamic acid production inMicrobiology (Reading, England) · 2024Article
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Engineering a Lactobacillus Lysine Riboswitch to Dynamically Control Metabolic Pathways for Lysine Production inMicroorganisms · 2024Article
- Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.Microbial cell factories · 2023Review
- Construction and Analysis of an Enzyme-Constrained Metabolic Model ofBiomolecules · 2022Article
- Protein engineering for feedback resistance in 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.Applied microbiology and biotechnology · 2022Article
- COSynthetic and systems biotechnology · 2022Article
- Development of a plasmid stabilization system in Vibrio natriegens for the high production of 1,3-propanediol and 3-hydroxypropionate.Bioresources and bioprocessing · 2021Article
- Metabolic Flux Analysis-Linking Isotope Labeling and Metabolic Fluxes.Metabolites · 2020Review
- Rational modification of the carbon metabolism of Corynebacterium glutamicum to enhance L-leucine production.Journal of industrial microbiology & biotechnology · 2020Article
- Rational modification of tricarboxylic acid cycle for improving L-lysine production in Corynebacterium glutamicum.Microbial cell factories · 2018Article
- Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose.Microbial cell factories · 2018Article
- Pyruvate cycle increases aminoglycoside efficacy and provides respiratory energy in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Engineering photosynthetic production of L-lysine.Metabolic engineering · 2017Article
- Systems metabolic engineering strategies for the production of amino acids.Synthetic and systems biotechnology · 2017Review
- Analysis of intracellular metabolites ofEngineering in life sciences · 2017Article
- Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum.Scientific reports · 2017Article
- Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli.Scientific reports · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Allosteric regulation of phosphoenolpyruvate carboxylase (PEPC) controls the metabolic flux distribution of anaplerotic pathways. In this study, the feedback inhibition of Corynebacterium glutamicum PEPC was rationally deregulated, and its effect on metabolic flux redistribution was evaluated. Based on rational protein design, six PEPC mutants were designed, and all of them showed significantly reduced sensitivity toward aspartate and malate inhibition. Introducing one of the point mutations (N917G) into the ppc gene, encoding PEPC of the lysine-producing strain C. glutamicum LC298, resulted in ∼37% improved lysine production. In vitro enzyme assays and (13)C-based metabolic flux analysis showed ca. 20 and 30% increases in the PEPC activity and corresponding flux, respectively, in the mutant strain. Higher demand for NADPH in the mutant strain increased the flux toward pentose phosphate pathway, which increased the supply of NADPH for enhanced lysine production. The present study highlights the importance of allosteric regulation on the flux control of central metabolism. The strategy described here can also be implemented to improve other oxaloacetate-derived products.
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