ArticleApplied and environmental microbiology2014
The third replicon of members of the Burkholderia cepacia Complex, plasmid pC3, plays a role in stress tolerance.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 41 citations in OpenAlex.
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- Colony morphotype variation inJournal of bacteriology · 2025Review
- Quorum sensing and DNA methylation play active roles in clinicalJournal of bacteriology · 2025Article
- Insights into the diverse roles of the terminal oxidases in Burkholderia cenocepacia H111.Scientific reports · 2025Article
- Transposon sequencing reveals the essential gene set and genes enabling gut symbiosis in the insect symbiontISME communications · 2024Article
- Independent origins and evolution of the secondary replicons of the class Gammaproteobacteria.Microbial genomics · 2023Article
- A universal stress protein upregulated by hypoxia has a role in Burkholderia cenocepacia intramacrophage survival: Implications for chronic infection in cystic fibrosis.MicrobiologyOpen · 2023Article
- Genomic features, antimicrobial susceptibility, and epidemiological insights intoFrontiers in cellular and infection microbiology · 2023Article
- Biosynthesis of fragin is controlled by a novel quorum sensing signal.Nature communications · 2018Article
- Key Players and Individualists of Cyclic-di-GMP Signaling inFrontiers in microbiology · 2018Article
- Article
- The Essential Genome of Burkholderia cenocepacia H111.Journal of bacteriology · 2017Article
- Subfunctionalization influences the expansion of bacterial multidrug antibiotic resistance.BMC genomics · 2017Article
- Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis.PLoS neglected tropical diseases · 2017Article
- Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection.Virulence · 2017Article
- Use of Synthetic Hybrid Strains To Determine the Role of Replicon 3 in Virulence of the Burkholderia cepacia Complex.Applied and environmental microbiology · 2017Article
- Complete Genome Sequences for Three Chromosomes of the Burkholderia stabilis Type Strain (ATCC BAA-67).Genome announcements · 2016Article
- Toxin-Antitoxin Systems in Clinical Pathogens.Toxins · 2016Review
- Orderly Replication and Segregation of the Four Replicons of Burkholderia cenocepacia J2315.PLoS genetics · 2016Article
- Characterization and Comparative Overview of Complete Sequences of the First Plasmids ofFrontiers in microbiology · 2016Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The metabolically versatile Burkholderia cepacia complex (Bcc) occupies a variety of niches, including the plant rhizosphere and the cystic fibrosis lung (where it is often fatal to the patient). Bcc members have multipartite genomes, of which the third replicon, pC3 (previously chromosome 3), has been shown to be a nonessential megaplasmid which confers virulence and both antifungal and proteolytic activity on several strains. In this study, pC3 curing was extended to cover strains of 16 of the 17 members of the Bcc, and the phenotypes conferred by pC3 were determined. B. cenocepacia strains H111, MCO-3, and HI2424 were previously cured of pC3; however, this had not proved possible in the epidemic strain K56-2. Here, we investigated the mechanism of this unexpected stability and found that efficient toxin-antitoxin systems are responsible for maintaining pC3 of strain K56-2. Identification of these systems allowed neutralization of the toxins and the subsequent deletion of K56-2pC3. The cured strain was found to exhibit reduced antifungal activity and was attenuated in both the zebrafish and the Caenorhabditis elegans model of infection. We used a PCR screening method to examine the prevalence of pC3 within 110 Bcc isolates and found that this replicon was absent in only four cases, suggesting evolutionary fixation. It is shown that plasmid pC3 increases the resistance of B. cenocepacia H111 to various stresses (oxidative, osmotic, high-temperature, and chlorhexidine-induced stresses), explaining the prevalence of this replicon within the Bcc.
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