ArticleJournal of immunological methods2014
Detection of immunoglobulin isotypes from dried blood spots.
Article in Journal of immunological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03106493 (Upstate New York Infant Development Screening Program), which is not on this map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Upstate New York Infant Development Screening Program
Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Maternal antenatal depression and offspring DNA methylation.Journal of affective disorders · 2026Article
- Multiplex Detection of Immunoglobulins Uncovers Intrathecal IgA Elevation in Multiple Sclerosis.Cells · 2026Article
- Farm exposure in infancy is associated with elevated systemic IgGScience translational medicine · 2025Article
- Serum-equivalency comparison, detection, and quantification of Group BHuman vaccines & immunotherapeutics · 2025Article
- Examination of newborn DNA methylation among women with polycystic ovary syndrome/hirsutism.Epigenetics · 2023Article
- A Toolkit for Monitoring Immunoglobulin G Levels from Dried Blood Spots of Patients with Primary Immunodeficiencies.Journal of clinical immunology · 2023Article
- Epigenetic gestational age and the relationship with developmental milestones in early childhood.Human molecular genetics · 2023Article
- Effects on neonatal immunoglobulin concentrations by infant mode of delivery in the upstate KIDS study (2008-2010).American journal of reproductive immunology (New York, N.Y. : 1989) · 2023Article
- Immunoglobulin adsorption and film formation on mechanically wrinkled and crumpled surfaces at submonolayer coverage.Nanoscale advances · 2023Article
- Observational
- Ecoimmunology in the field: Measuring multiple dimensions of immune function with minimally invasive, field-adapted techniques.American journal of human biology : the official journal of the Human Biology Council · 2022Review
- Exposure to perfluoroalkyl substances and neonatal immunoglobulin profiles in the upstate KIDS study (2008-2010).Environmental pollution (Barking, Essex : 1987) · 2022Article
- Immunoglobulin G passive transfer from mothers to infants: total IgG, IgG subclasses and specific antipneumococcal IgG in 6-week Malawian infants exposed or unexposed to HIV.BMC infectious diseases · 2022Article
- Conception by fertility treatment and offspring deoxyribonucleic acid methylation.Fertility and sterility · 2021Article
- Evaluation of Circulating Cardiovascular Biomarker Levels for Early Detection of Congenital Heart Disease in Newborns in Sweden.JAMA network open · 2020Article
- Dengue in Western Uganda: a prospective cohort of children presenting with undifferentiated febrile illness.BMC infectious diseases · 2020Observational
- Dried Blood Spot in Laboratory: Directions and Prospects.Diagnostics (Basel, Switzerland) · 2020Review
- Serologic Tools and Strategies to Support Intervention Trials to Combat Zika Virus Infection and Disease.Tropical medicine and infectious disease · 2019Review
- Concentrations of endocrine disrupting chemicals in newborn blood spots and infant outcomes in the upstate KIDS study.Environment international · 2018Article
- Newborn adipokines and early childhood growth.Pediatric obesity · 2018Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
The study was designed to determine the sensitivity and reproducibility of recovering immunoglobulin (Ig) isotypes (IgG subclasses, IgA, IgE and IgM classes) from dried blood spots (DBS), a methodologic subcomponent of the Upstate KIDS Study. A multiplexed Luminex assay was used for IgG1/2/3/4, IgA and IgM analysis; an ELISA was used for IgE. Plasma samples from de-identified patients were used to compare the Luminex assay with nephelometry, which is routinely used to quantify IgA, IgG and IgM in clinical samples. The IgE ELISA was compared to an immunofluorescence assay. Prior to evaluation of punches from newborn dried blood spots (NDBSs), recoveries of Ig from punches of cord blood DBSs (CBDBSs) vs. plasma from the same cord bloods were compared. Although the recoveries of Ig from plasma and DBSs were not comparable, which could be due to cell lysates in the DBS samples, the analyses were reproducible. Additionally, the levels of IgA, IgG2, IgG4, and IgM recovered from CBDBSs positively correlated with those in plasma. The DBS data is a relative value since it is not equivalent to the plasma concentration. The majority of Ig concentrations recovered from 108 newborns of the Upstate KIDs Study were within the range of newborn plasma Ig levels with the exception of IgG3. The IgG4 values displayed the greatest variance with a wide range (0.01-319 mg/dl), whereas, IgG1 values had the narrowest range (85.2-960.4 mg/dl).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.