ArticleParasites & vectors2013
Proteomic analysis of surface proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 65 citations in OpenAlex.
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- Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Genome-Wide Identification and Characterization of Major RNAi Genes Highlighting Their Associated Factors in Cowpea (BioMed research international · 2023Article
- Evaluation of larval surface antigens from infective larvae of Strongyloides venezuelensis for the serodiagnosis of human strongyloidiasis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2023Article
- Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice.PLoS neglected tropical diseases · 2022Article
- Immunoproteomic Analysis ofPathogens (Basel, Switzerland) · 2022Article
- The Use of MALDI-TOF MS as a Diagnostic Tool for AdultFrontiers in veterinary science · 2022Article
- Vaccines as a Strategy to Control Trichinellosis.Frontiers in microbiology · 2022Review
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- TheFrontiers in cellular and infection microbiology · 2021Article
- Galectin-Receptor Interactions Regulates Cardiac Pathology Caused byFrontiers in immunology · 2021Article
- Molecular cloning and characterization of a novel peptidase from Trichinella spiralis and protective immunity elicited by the peptidase in BALB/c mice.Veterinary research · 2020Article
- The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis.Parasites & vectors · 2020Article
- Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected withPathogens (Basel, Switzerland) · 2020Article
- Proteomic analysis of excretory-secretory products from young adults of Angiostrongylus cantonensis.Memorias do Instituto Oswaldo Cruz · 2019Article
- Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice.Veterinary research · 2018Article
- Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting.Parasites & vectors · 2018Article
- Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA.Veterinary research · 2018Article
- Molecular characterization of Trichinella spiralis galectin and its participation in larval invasion of host's intestinal epithelial cells.Veterinary research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrichinella spiralis is a zoonotic tissue-dwelling parasitic nematode that infects humans and other mammals. Its surface proteins are recognized as antigenic in many infected hosts, being directly exposed to the host's immune system and are the main target antigens that induce the immune responses. The larval surface proteins may also interact with intestinal epithelial cells and may play an important role in the invasion and development process of T. spiralis. The purpose of this study was to analyze and characterize the surface proteins of T. spiralis muscle larvae by two-dimensional gel electrophoresis (2-DE) and mass spectrometry.
methodsThe surface proteins of T. spiralis muscle larvae were stripped from the cuticle of live larvae by the cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate. The surface protein stripping was examined by an immunofluorescent test (IFT). The surface proteins were analyzed by SDS-PAGE and Western blotting, and then identified by 2-DE and MALDI-TOF/TOF mass spectrometry analysis.
resultsThe IFT results showed that the surface proteins-stripped larvae were not recognized by sera of mice immunized with surface antigens. Western blotting showed 7 of 12 protein bands of the surface proteins were recognized by mouse infection sera at 18 dpi and at 42 dpi. The 2-DE results showed that a total of approximately 33 proteins spots were detected with molecular weights varying from 10 to 66 kDa and isoelectric point (pI) from 4 to 7. Twenty-seven of 33 protein spots were identified and characterized to correlate with 15 different proteins. Out of the 14 proteins identified as T. spiralis proteins, 5 proteins (partial P49 antigen, deoxyribonuclease II family protein, two serine proteases, and serine proteinase) had catalytic and hydrolase activity. All of these 5 proteins were also associated with metabolic processes and 2 of the five proteins were associated with cellular processes.
conclusionsIn this study, T. spiralis muscle larval surface proteins have been identified, which will provide useful information to elucidate the host-parasite interaction, identify the invasion-related proteins, early diagnostic antigens and the targets for a vaccine.
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